Programmed Flagellar Ejection in Caulobacter crescentus Leaves PL-subcomplexes.
Programmed Flagellar Ejection in Caulobacter crescentus Leaves PL-subcomplexes.
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DOI：
10.1016/j.jmb.2021.167004
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发表时间：
2021-06-25
影响因子：
5.6
通讯作者：
Jensen, Grant J.
中科院分区：
文献类型：
--
作者：
Kaplan, Mohammed;Wang, Yuhang;Chreifi, Georges;Zhang, Lujia;Chang, Yi-Wei;Jensen, Grant J.
细菌鞭毛由一条长的细胞外细丝组成，该细丝通过嵌入细胞包膜中的马达旋转。虽然鞭毛装配已被广泛研究，拆卸过程仍然不太清楚。除了在新月柄杆菌的生命周期中发生的程序化鞭毛弹射外，我们和其他人最近还表明，许多细菌物种在饥饿条件下失去鞭毛，在外膜中留下遗迹结构。然而，C. crescentus留下类似的遗物或没有。在这里，我们对C. crescentus的生命周期，使用电子冷冻断层扫描（cryo-ET），并发现鞭毛遗迹亚复合物，类似于那些在饥饿诱导的过程中产生的，仍然作为鞭毛喷射在细胞发育过程中的结果。这种相似性表明C. crescentus可能与更普遍的，可能更古老的，与饥饿有关的鞭毛丧失共享一条共同的进化路径。
The bacterial flagellum consists of a long extracellular filament that is rotated by a motor embedded in the cell envelope. While flagellar assembly has been extensively studied, the disassembly process remains less well understood. In addition to the programmed flagellar ejection that occurs during the life cycle of Caulobacter crescentus, we and others have recently shown that many bacterial species lose their flagella under starvation conditions, leaving relic structures in the outer membrane. However, it remains unknown whether the programmed flagellar ejection of C. crescentus leaves similar relics or not. Here, we imaged the various stages of the C. crescentus life cycle using electron cryo-tomography (cryo-ET) and found that flagellar relic subcomplexes, akin to those produced in the starvation-induced process, remain as a result of flagellar ejection during cell development. This similarity suggests that the programmed flagellar ejection of C. crescentus might share a common evolutionary path with the more general, and likely more ancient, starvation-related flagellar loss.
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影响因子：
3
作者：
Kremer, JR;Mastronarde, DN;McIntosh, JR
通讯作者：
McIntosh, JR
影响因子：
3.6
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Bai, Fan
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Mastronarde, DN
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Chreifi, Georges;Chen, Songye;Jensen, Grant J.
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Jensen, Grant J.
影响因子：
3.6
作者：
Aldridge, P;Jenal, U
通讯作者：
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