Expression, purification, and analysis of recombinant Drosophila Dicer-1 and Dicer-2 enzymes.
Expression, purification, and analysis of recombinant Drosophila Dicer-1 and Dicer-2 enzymes.
复制标题
DOI：
10.1007/978-1-59745-191-8_2
复制
发表时间：
2008
影响因子：
--
通讯作者：
Xuecheng Ye;Qinghua Liu
中科院分区：
文献类型：
--
作者：
Xuecheng Ye;Qinghua Liu
RNA干扰（RNA interference，RNAi）是一种由微小RNA（microRNA，miRNA）和小干扰RNA（small interfering RNA，siRNA）介导的转录后基因沉默。在果蝇中，RNase III酶Dicer-1和Dicer-2分别产生miRNA和siRNA。我们描述了重组Dicer-1和Dicer-2酶的表达、纯化和分析方法。我们的研究表明，Dicer-1和Dicer-2显示不同的底物特异性和ATP需求。
RNA interference (RNAi) is a form of posttranscriptional gene silencing mediated by microRNA (miRNA) and small interfering RNA (siRNA). InDrosophila melanogaster, the RNase III enzymes Dicer-1 and Dicer-2 generate miRNA and siRNA, respectively. We describe the methods for the expression, purification, and analysis of recombinant Dicer-1 and Dicer-2 enzymes. Our studies demonstrate that Dicer-1 and Dicer-2 display different substrate specificities and ATP requirements.