Ink and Vinegar, a Simple Staining Technique for Arbuscular-Mycorrhizal Fungi
Ink and Vinegar, a Simple Staining Technique for Arbuscular-Mycorrhizal Fungi
复制标题
DOI：
10.1128/aem.64.12.5004-5007.1998
复制
发表时间：
1998-12
影响因子：
4.4
通讯作者：
H. Vierheilig;Andrew P. Coughlan;U. Wyss;Y. Piché
中科院分区：
文献类型：
--
作者：
H. Vierheilig;Andrew P. Coughlan;U. Wyss;Y. Piché
摘要我们开发了一种可靠、廉价、简单的方法来染色根组织中的丛枝菌根真菌定殖。除了在研究中的应用，这种无毒，高质量的染色方法也可以在教学练习中发挥很大的作用。在用KOH充分清洁后，墨水醋溶液成功地将所有真菌结构染色，使它们清晰可见。
ABSTRACT We developed a reliable, inexpensive, and simple method for staining arbuscular-mycorrhizal fungal colonizations in root tissues. Apart from applications in research, this nontoxic, high-quality staining method also could be of great utility in teaching exercises. After adequate clearing with KOH, an ink-vinegar solution successfully stained all fungal structures, rendering them clearly visible.