ROCC, a conserved region in cohesin's Mcd1 subunit, is essential for the proper regulation of the maintenance of cohesion and establishment of condensation.
ROCC, a conserved region in cohesin's Mcd1 subunit, is essential for the proper regulation of the maintenance of cohesion and establishment of condensation.
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DOI：
10.1091/mbc.e14-04-0929
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发表时间：
2014-08-15
影响因子：
3.3
通讯作者：
Koshland D
中科院分区：
文献类型：
--
作者：
Eng T;Guacci V;Koshland D
A novel evolutionarily conserved motif termed ROCC (regulation of cohesion and condensation) is identified in the regulatory subunit of cohesin. This study offers important insights into the regulation of the maintenance of cohesion and condensation and the molecular basis for cohesin's chromatin-tethering activity. Cohesin helps orchestrate higher-order chromosome structure, thereby promoting sister chromatid cohesion, chromosome condensation, DNA repair, and transcriptional regulation. To elucidate how cohesin facilitates these diverse processes, we mutagenized Mcd1p, the kleisin regulatory subunit of budding yeast cohesin. In the linker region of Mcd1p, we identified a novel evolutionarily conserved 10–amino acid cluster, termed the regulation of cohesion and condensation (ROCC) box. We show that ROCC promotes cohesion maintenance by protecting a second activity of cohesin that is distinct from its stable binding to chromosomes. The existence of this second activity is incompatible with the simple embrace mechanism of cohesion. In addition, we show that the ROCC box is required for the establishment of condensation. We provide evidence that ROCC controls cohesion maintenance and condensation establishment through differential functional interactions with Pds5p and Wpl1p.
