BRN-3.0 - A POU-DOMAIN PROTEIN EXPRESSED IN THE SENSORY, IMMUNE, AND ENDOCRINE SYSTEMS THAT FUNCTIONS ON ELEMENTS DISTINCT FROM KNOWN OCTAMER MOTIFS
BRN-3.0 - A POU-DOMAIN PROTEIN EXPRESSED IN THE SENSORY, IMMUNE, AND ENDOCRINE SYSTEMS THAT FUNCTIONS ON ELEMENTS DISTINCT FROM KNOWN OCTAMER MOTIFS
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DOI：
10.1073/pnas.90.22.10841
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发表时间：
1993-11-15
影响因子：
11.1
通讯作者：
ROSENFELD, MG
中科院分区：
文献类型：
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作者：
GERRERO, MR;MCEVILLY, RJ;ROSENFELD, MG
Brn-3.0的表征和IV类POU结构域家族的高度相关成员（Brn-3.1）的鉴定表明Brn-3.0在视网膜神经节细胞和感觉神经元的发育中的潜在作用，以及在脑垂体和免疫系统中的潜在作用。Brn-3.0在脑垂体和促肾上腺皮质激素细胞系中表达。一个功能性的DNA反应元件已被确定在proopiomelanocortin启动子。与先前描述的哺乳动物POU结构域蛋白相比，Brn-3.0相对无效地结合到已知的八聚体DNA基序，而是以高亲和力结合到一组不同的DNA元件，作为转录激活因子发挥作用。Brn-3.0、Brn-3.1和果蝇tI-POU共享一个N-末端同源区域，称为“POU-IV盒”，其类似于c-myc基因家族中的保守功能结构域。
Characterization of Brn-3.0 and identification of a highly related member (Brn-3.1) of the class IV POU-domain family suggest potential roles of Brn-3.0 in the development of retinal ganglion cells and sensory neurons, as well as potential roles in the pituitary gland and the immune system. Brn-3.0 is expressed in the pituitary gland and in a corticotroph cell line. A functional DNA response element has been identified in the proopiomelanocortin promoter. In contrast to previously described mammalian POU-domain proteins, Brn-3.0 binds relatively ineffectively to known octamer DNA motifs, but instead binds with high affinity to a distinct set of DNA elements, functioning as a transcriptional activator. Brn-3.0, Brn-3.1, and the Drosophila tI-POU share an N-terminal region of homology, referred to as the ''POU-IV box,'' which is similar to a conserved functional domain in the c-myc gene family.