Identification of a CYP3A-like gene and CYPs mRNA expression modulation following exposure to benzo[a]pyrene in the bivalve mollusk Chlamys farreri.
Identification of a CYP3A-like gene and CYPs mRNA expression modulation following exposure to benzo[a]pyrene in the bivalve mollusk Chlamys farreri.
复制标题
DOI：
10.1016/j.marenvres.2013.11.001
复制
发表时间：
2014-03
影响因子：
3.3
通讯作者：
Shuangmei Tian;L. Pan;Hui Zhang
中科院分区：
文献类型：
--
作者：
Shuangmei Tian;L. Pan;Hui Zhang
本研究从栉孔扇贝卵巢中克隆了一个CYP3A类似基因。CYP3A样基因在消化腺和性腺中表达水平较高，提示其在类固醇和外源性物质代谢中的作用。扇贝暴露于多环芳烃（PAHs）、苯并[a]芘（B [a] P）中10 d后，测定其体内的PAHs浓度。B [a] P暴露10 d后，可剂量依赖性上调CYP4和CYP3A样基因。而10 μ g/L B [a] P组则未见CYP3A样活性的诱导。0.5和10 μ g/L B [a] P可引起DNA损伤，10 μ g/L B [a] P还可引起氧化损伤。这些结果表明，环境污染物对贝类CYP具有一定的调节作用，环境污染物的高遗传毒性和氧化损伤可能是导致贝类CYP 3A和CYP 1A表达受阻的部分原因。
In this study, we isolated a CYP3A-like gene from ovary of the scallop (Chlamysfarreri). High levels of CYP3A-like gene expression occur in the digestive gland and gonad, which suggested their role in the metabolism of steroids and xenobiotics. Scallops were exposed to a polycyclic aromatic hydrocarbons (PAHs), benzo[a]pyrene (B[a]P) for 10 days. The CYP4 and CYP3A-like gene can be up-regulated by B[a]P in a dose-dependent manner after 10 days exposure. But no induction of the CYP3A-like was observed in 10 μg/L B[a]P group. The CYP1A-like expression can only be induced by 0.025 μg/L B[a]P. 0.5 and 10 μg/L B[a]P caused significant DNA damage and 10 μg/L B[a]P can also lead to oxidative damage. These results demonstrate that the mollusk CYPs can be modulated by environmental pollutant, and the blocked induction of CYP3A-like and CYP1A-like expression probably results from the high genotoxicity and oxidative damage partly.