A direct nanoflow liquid chromatography - Tandem system for interaction proteomics
A direct nanoflow liquid chromatography - Tandem system for interaction proteomics
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DOI：
10.1021/ac020018n
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发表时间：
2002-09-15
影响因子：
7.4
通讯作者：
Isobe, T
中科院分区：
文献类型：
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作者：
Natsume, T;Yamauchi, Y;Isobe, T
功能蛋白质组学的研究目的是在蛋白质水平上发现基因功能，功能蛋白质组学的策略之一是全面分析与蛋白质之间功能联系相关的蛋白质-蛋白质相互作用，并分析功能细胞机制以更好地了解细胞功能的基础。在这里，我们描述了直接纳米流LC(DNLC)系统，它配备了一个填充了1微米反相(RP)珠子的无熔块高分辨率电喷雾界面柱，以及一个新型的无分离式纳米流梯度洗脱系统来操作该柱。在极慢的流速下使用RP-DNLC，
One of the strategies of functional proteomics, research aiming to discover gene function at the protein level, is the comprehensive analysis of protein-protein interactions related to the functional linkage among proteins and analysis of functional cellular machinery to better understand the basis of cell functions. Here, we describe the direct nanoflow LC (DNLC) system, which is equipped with a fritless high-resolution electrospray interface column packed with 1-mum reversed-phase (RP) beads and a novel splitless nanoflow gradient elution system to operate the column. Using RP-DNLC at an extremely slow flow rate,