Preparation of silica hydrogels using a synthetic peptide for application as carriers for controlled drug release and mesoporous oxides
Preparation of silica hydrogels using a synthetic peptide for application as carriers for controlled drug release and mesoporous oxides
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DOI：
10.2109/jcersj2.122.134
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发表时间：
2014-02
影响因子：
1.1
通讯作者：
Y. Kawachi;Shin-ichi Kugimiya;Katsuya Kato
中科院分区：
文献类型：
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作者：
Y. Kawachi;Shin-ichi Kugimiya;Katsuya Kato
溶胶-凝胶法是一种常用的酶和药物的包封方法，但该方法具有使用酸或碱作为催化剂和难以控制二氧化硅材料孔径的两个缺点。尽管已经报道了在温和条件下合成或在中孔区域具有受控孔径的二氧化硅，但仍然难以同时实现这两个特征。本研究选择赖氨酸（K）、组氨酸（H）、封闭和交替的K和天冬氨酸（D）等10肽作为硅矿化的催化剂，采用“先静置“合成法制备了硅胶。将所得二氧化硅水凝胶冻干，并分别使用Brunauer BET法和场发射扫描电子显微镜（FE-SEM）表征其表面积和形态。采用“静置法“制备的硅胶具有较高的比表面积（分别为576和451 m2 g <$1）和孔容（分别为0.35和0.30 cm 3 g <$1）。SEM图像证实了这些凝胶的介孔结构。我们以K10和H10为催化剂，将黄绿素钠盐作为模型药物包封在硅胶水凝胶中。使用H10制备的二氧化硅水凝胶显示出比使用K10制备的凝胶更快的药物释放（约2.5倍）。这些结果表明，通过改变肽与孔结构之间的等电点结合，可以设计合成的二氧化硅水凝胶-肽复合材料来控制肽的释放速率。
Sol gel methods is a commonly used methods for encapsulation of enzyme and drug, but this method has two disadvantages of using acid or base as a catalyst and being dif fi cult to control pore size of silica material. Even though synthesis under mild condition or silica with controlled pore size in the mesopore region have been reported, it is still dif fi cult to achieve these two characteristics simultaneously. In this work, we chose 10-mer peptides of lysine (K), histidine (H), and block and alternate K and aspartic acid (D) as catalysts for silica mineralization, and silica gels were prepared using the synthetic peptides and a “ leave to stand ” synthesis method. The resulting silica hydrogels were lyophilized, and their surface areas and morphologies were characterized using the Brunauer Emmett Teller (BET) method and fi eld-emission scanning electron microscopy (FE-SEM), respectively. Silica gels prepared by the “ leave to stand ” method with K 10 and H 10 exhibited a mesoporous structure with high surface area (576 and 451m 2 g ¹ 1 , respectively) and pore volume (0.35 and 0.30cm 3 g ¹ 1 , respectively). SEM images con fi rmed the mesoporous structure of these gels. We encapsulated fl uorescein sodium salt as a model drug within silica hydrogels using K 10 and H 10 as a catalyst. The silica hydrogel prepared using H 10 exhibited faster release of the drug (approximately 2.5-fold) than gels prepared using K 10 . These results demonstrate that by changing isoelectric point binding between the peptide and pore structure, the synthesized silica hydrogel-peptide composites can be designed to control the release rate of