Suppressive effect of insulin infusion on chemokines and chemokine receptors.
Suppressive effect of insulin infusion on chemokines and chemokine receptors.
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DOI：
10.2337/dc09-2193
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发表时间：
2010-05
期刊：
影响因子：
16.2
通讯作者：
Dandona P
中科院分区：
文献类型：
--
作者：
Ghanim H;Korzeniewski K;Sia CL;Abuaysheh S;Lohano T;Chaudhuri A;Dandona P
鉴于胰岛素的抗炎作用，我们研究了胰岛素对单核细胞（MNCs）中趋化因子（单核细胞趋化蛋白-1（MCP-1）和正常T细胞表达和分泌的活化调节因子（RANTES）及其受体（趋化因子受体（CCR）-2和CCR-5）的血浆浓度和表达的潜在抑制作用。我们还研究了胰岛素对其他趋化因子的影响。10例肥胖2型糖尿病患者以2 U/h输注5%葡萄糖100 ml/h，持续4 h。另设对照组8例和2型糖尿病患者6例，分别以5%葡萄糖100 ml/h或生理盐水100 ml/h输注4 h。在0、2、4和6 h采集血样。胰岛素输注可显著抑制单核细胞中MCP-1、eotaxin和RANTES的血浆浓度以及RANTES、巨噬细胞炎性蛋白（MIP）-1β、CCR-2和CCR-5的表达。葡萄糖和生理盐水输注没有改变这些指标。低剂量胰岛素输注抑制了关键趋化因子MCP-1和RANTES的血浆浓度，以及它们各自的受体CCR-2和CCR-5在MNC中的表达。胰岛素还抑制RANTES和MIP-1β在MNCs中的表达。这些作用可能有助于胰岛素的全面抗炎作用。
In view of the previously described anti-inflammatory effects of insulin, we investigated the potential suppressive effect of insulin on plasma concentrations and expression of the chemokines, monocyte chemoattractant protein-1 (MCP-1) and regulated on activation normal T-cell expressed and secreted (RANTES) and their receptors, chemokine receptor (CCR)-2 and CCR-5, in mononuclear cells (MNCs). We also investigated the effect of insulin on other chemokines. Ten obese type 2 diabetic patients were infused with insulin (2 units/h with 100 ml of 5% dextrose/h) for 4 h. Another 8 and 6 type 2 diabetic patients were infused with 100 ml of 5% dextrose/h or saline for 4 h, respectively, and served as control subjects. Blood samples were obtained at 0, 2, 4, and 6 h. Insulin infusion significantly suppressed the plasma concentrations of MCP-1, eotaxin, and RANTES and the expression of RANTES, macrophage inflammatory protein (MIP)-1β, CCR-2, and CCR-5 in MNCs at 2 and 4 h. Dextrose and saline infusions did not alter these indexes. A low-dose infusion of insulin suppresses the plasma concentration of key chemokines, MCP-1, and RANTES, and the expression of their respective receptors, CCR-2 and CCR-5, in MNCs. Insulin also suppresses the expression of RANTES and MIP-1β in MNCs. These actions probably contribute to the comprehensive anti-inflammatory effect of insulin.
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影响因子：
56.9
作者：
COCCHI, F;DEVICO, AL;LUSSO, P
通讯作者：
LUSSO, P
影响因子：
5.8
作者：
Aljada, A;Ghanim, H;Dandona, P
通讯作者：
Dandona, P
影响因子：
5.8
作者：
Bonds, Denise E.;Larson, Joseph C.;Margolis, Karen L.
通讯作者：
Margolis, Karen L.
DOI：
10.1161/atvbaha.108.180505
发表时间：
2009-10
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Gautier EL;Jakubzick C;Randolph GJ
通讯作者：
Randolph GJ
影响因子：
15.3
作者：
JOSE, PJ;GRIFFITHSJOHNSON, DA;WILLIAMS, TJ
通讯作者：
WILLIAMS, TJ