Studies on plasminogen. VIII. Species specificity of streptokinase
Studies on plasminogen. VIII. Species specificity of streptokinase
复制标题
纤溶酶原的研究。
DOI：
10.1139/o69-145
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发表时间：
1969
影响因子：
2.9
通讯作者：
E. Mertz
中科院分区：
文献类型：
--
作者：
R. Wulf;E. Mertz
用连续流动电泳法从10种动物血清中分离纯化纤溶酶原。在以下动物中，以尿激酶为激活剂的纯化纤溶酶原中的酯溶活性与酪蛋白溶解活性的比值为：大鼠9.8、牛6.6、猪5.7、猫3.5、绵羊3.4、小鼠3.3、兔2.7、人2.2、猴2.0和狗1.4。用链激酶激活纤溶酶原将物种分为三组：（a）用少量链激酶激活（人、猫、猴），（B）用大量链激酶激活（犬、兔），和（c）未激活（牛、绵羊、猪、小鼠和大鼠）。链激酶-人球蛋白混合物和尿激酶同等程度地激活所有纤溶酶原。当十个物种中的九个物种的血清的优球蛋白组分在pH 2.5下进行淀粉凝胶电泳时，a组和B组给出两条主要纤溶酶原条带，而c组仅给出一条。
Continuous-flow electrophoresis was used to isolate purified plasminogens from the serums of 10 species. The ratios of esterolytic to caseinolytic activity in the purified plasminogens with urokinase as the activator for the following animals were: rat 9.8, cow 6.6, pig 5.7, cat 3.5, sheep 3.4, mouse 3.3, rabbit 2.7, man 2.2, monkey 2.0, and dog 1.4. Plasminogen activation with streptokinase divides the species into three groups: (a) activated with small amounts of streptokinase (man, cat, monkey), (b) activated with large amounts of streptokinase (dog, rabbit), and (c) not activated (cow, sheep, pig, mouse, and rat). Streptokinase – human globulin mixture and urokinase activated all plasminogens equally well When the euglobulin fractions of the serums of nine of the ten species were subjected to starch gel electrophoresis at pH 2.5, groups a and b gave two major plasminogen bands, whereas c gave only one.