Cyanobacterial response regulator PatA contains a conserved N-terminal domain (PATAN) with an alpha-helical insertion
Cyanobacterial response regulator PatA contains a conserved N-terminal domain (PATAN) with an alpha-helical insertion
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DOI：
10.1093/bioinformatics/btl096
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发表时间：
2006-06-01
期刊：
影响因子：
5.8
通讯作者：
Galperin, Michael Y.
中科院分区：
文献类型：
--
作者：
Makarova, Kira S.;Koonin, Eugene V.;Galperin, Michael Y.
蓝藻鱼腥藻（Nostoc）PCC 7120通过将丝状体中大约每10个细胞分化成称为异形胞的固氮细胞来响应氮化合物的饥饿。异形胞的形成受到复杂的调控，其中涉及一种不寻常的反应调节剂PatA，其C-末端含有Chey样磷酸受体（受体，REC）结构域。PatA类反应调节剂广泛存在于蓝藻中，其中之一调节集胞藻PCC 6803的趋光性。PatA的序列分析显示，除了REC结构域，以前未检测到的，保守的结构域，我们命名为PATAN（PatA N-末端后），和一个潜在的螺旋-转角-螺旋（HTH）结构域。PATAN结构域在多种环境细菌和古细菌中编码，通常每个基因组有几个拷贝，并且通常与REC、路障和其他信号转导结构域或与DNA结合HTH结构域相关。许多PATAN结构域包含一个小的额外结构域的插入，称为a-剪辑，预计形成一个四螺旋束。PATAN结构域似乎参与蛋白质-蛋白质相互作用，其调节蓝细菌和一些蛋白细菌（如黄色粘球菌和硫还原地芽孢杆菌）中的滑行运动和细胞发育和分化过程。
The cyanobacterium Anabaena (Nostoc) PCC 7120 responds to starvation for nitrogen compounds by differentiating approximately every 10th cell in the filament into nitrogen-fixing cells called heterocysts. Heterocyst formation is subject to complex regulation, which involves an unusual response regulator PatA that contains a CheY-like phosphoacceptor (receiver, REC) domain at its C-terminus. PatA-like response regulators are widespread in cyanobacteria; one of them regulates phototaxis in Synechocystis PCC 6803. Sequence analysis of PatA revealed, in addition to the REC domain, a previously undetected, conserved domain, which we named PATAN (after PatA N-terminus), and a potential helix-turn-helix (HTH) domain. PATAN domains are encoded in a variety of environmental bacteria and archaea, often in several copies per genome, and are typically associated with REC, Roadblock and other signal transduction domains, or with DNA-binding HTH domains. Many PATAN domains contain insertions of a small additional domain, termed a-clip, which is predicted to form a four-helix bundle. PATAN domains appear to participate in protein-protein interactions that regulate gliding motility and processes of cell development and differentiation in cyanobacteria and some proteobacteria, such as Myxococcus xanthus and Geobacter sulfurreducens.