Characterization of a 40S ribosomal subunit complex in polyribosomes of Saccharomyces cerevisiae treated with cycloheximide
Characterization of a 40S ribosomal subunit complex in polyribosomes of Saccharomyces cerevisiae treated with cycloheximide
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用放线菌酮处理的酿酒酵母多聚核糖体中 40S 核糖体亚基复合物的表征
DOI：
10.1128/mcb.1.1.51-57.1981
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发表时间：
1981
影响因子：
5.3
通讯作者：
A. E. Dahlberg
中科院分区：
文献类型：
--
作者：
T. Helser;Robert A. Baan;A. E. Dahlberg
在特定条件下，放线菌酮处理酿酒酵母引起一种称为“半聚体”的多聚核糖体的积累。“半聚体的有限核糖核酸酶消化从多聚核糖体中释放出被鉴定为40S核糖体亚基的颗粒。数据表明，半聚体是含有附加的40S核糖体亚基的多聚核糖体，其连接到信使核糖核酸。半聚体的蛋白质凝胶电泳分析显示许多非核糖体蛋白。其中两种蛋白质与酵母起始因子eIF2的亚基共迁移。
Under specific conditions cycloheximide treatment of Saccharomyces cerevisiae caused the accumulation of a type of polyribosome called "halfmer." Limited ribonuclease digestion of halfmers released particles from the polyribosomes identified as 40S ribosomal subunits. The data demonstrated that halfmers are polyribosomes containing an additional 40S ribosomal subunit attached to the messenger ribonucleic acid. Protein gel electrophoretic analysis of halfmers revealed numerous nonribosomal proteins. Two of these proteins comigrate with subunits of yeast initiation factor eIF2.