Phage infection of the obligate intracellular bacterium, Chlamydia psittaci strain guinea pig inclusion conjunctivitis
Phage infection of the obligate intracellular bacterium, Chlamydia psittaci strain guinea pig inclusion conjunctivitis
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DOI：
10.1016/s1286-4579(00)90356-3
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发表时间：
2000-06-01
影响因子：
5.8
通讯作者：
Bavoil, PM
中科院分区：
文献类型：
--
作者：
Hsia, RC;Ohayon, H;Bavoil, PM
利用噬菌体超感染、衣原体感染的HeLa细胞的透射电镜观察了phcpg1（一种感染专性细胞内病原体鹦鹉热衣原体豚鼠包涵性结膜炎的噬菌体）的感染周期。证明了噬菌体附着于细胞外、代谢休眠、感染性初级体和共内化。进入后，一旦初级体分化为代谢活跃的网状体，噬菌体感染就会发生。噬菌体感染的细菌遵循一种改变的发育路径，即细胞分裂被抑制，产生异常大的网状体，称为大网状体，不能成熟为初级体。这些形式最终在衣原体发育周期的后期裂解，在包涵体中释放大量的噬菌体后代，并在包涵膜裂解后进入宿主细胞的细胞质。高度感染的HeLa细胞的结构完整性在晚期明显受损。释放的噬菌体颗粒在不同发育阶段热切地附着在裂解和未裂解的衣原体外膜的外小叶上，表明在衣原体发育周期中，特定的噬菌体受体均匀地存在于外膜上。提出了噬菌体感染的一种机制，即噬菌体通过附着在具有传染性的初级体上，从而获得复制衣原体的途径，随后破坏衣原体的发育周期以满足自身的复制需要。噬菌体感染的意义在衣原体感染和疾病的背景下进行了讨论。(C) 2000年版《科学与医学》Elsevier SAS。
The infectious cycle of phi CPG1, a bacteriophage that infects the obligate intracellular pathogen, Chlamydia psittaci strain Guinea Pig Inclusion Conjunctivitis, was observed using transmission electron microscopy of phage-hyperinfected, Chlamydia-infected HeLa cells. Phage attachment to extracellular, metabolically dormant, infectious elementary bodies and cointernalisation are demonstrated. Following entry, phage infection takes place as soon as elementary bodies differentiate into metabolically active reticulate bodies. Phage-infected bacteria follow an altered developmental path whereby cell division is inhibited, producing abnormally large reticulate bodies, termed maxi-reticulate bodies, which do not mature to elementary bodies. These forms eventually lyse late in the chlamydial developmental cycle, releasing abundant phage progeny in the inclusion and, upon lysis of the inclusion membrane, into the cytosol of the host cell. Structural integrity of the hyperinfected HeLa cell is markedly compromised at late stages. Released phage particles attach avidly to the outer leaflet of the outer membranes of lysed and unlysed Chlamydiae at different stages of development, suggesting the presence of specific phage receptors in the outer membrane uniformly during the chlamydial developmental cycle. A mechanism for phage infection is proposed, whereby Phage gains access to replicating chlamydiae by attaching to the infectious elementary body, subsequently subverting the: chlamydial developmental cycle to its own replicative needs. The implications of phage infection in the context of chlamydial infection and disease are discussed. (C) 2000 Editions scientifiques et medicales Elsevier SAS.