A Genomic View on Nitrogen Metabolism and Nitrogen Control in Mycobacteria
A Genomic View on Nitrogen Metabolism and Nitrogen Control in Mycobacteria
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DOI：
10.1159/000159195
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发表时间：
2009-01-01
影响因子：
1.2
通讯作者：
Burkovski, Andreas
中科院分区：
文献类型：
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作者：
Amon, Johannes;Titgemeyer, Fritz;Burkovski, Andreas
关于分枝杆菌属中氮代谢和控制的知识很少，特别是与相关放线菌如天蓝色链霉菌或近亲谷氨酸棒杆菌的知识状态相比。因此，我们筛选了已发表的耻垢分枝杆菌、结核分枝杆菌、牛分枝杆菌、鸟分枝杆菌ssp.副结核杆菌和麻风分枝杆菌的基因编码蛋白质的吸收氮源，氮同化和氮控制系统，导致在一个详细的比较基因组分析的氮代谢相关基因的属的所有完全测序的成员。可以确定铵、硝酸盐和尿素的转运蛋白，以及对这些氮源的同化至关重要的酶，即谷氨酰胺合成酶、谷氨酸脱氢酶、谷氨酸合成酶、硝酸还原酶、亚硝酸还原酶和尿素酶蛋白。在致病分枝杆菌中，尤其是在分枝杆菌中，编码氮转运和代谢蛋白的基因减少。麻风病人不同物种的信号转导成分包括腺苷酰和尿苷酰转移酶和P-II型信号转导蛋白。只为M。在分枝杆菌中，发现了两个可能的氮调节蛋白的同源物，即GlnR和AmtR，而在其他分枝杆菌中，AmtR不存在，GlnR似乎是氮转录调节蛋白。版权所有（C）2008 S. Karger AG，巴塞尔
Knowledge about nitrogen metabolism and control in the genus Mycobacterium is sparse, especially compared to the state of knowledge in related actinomycetes like Streptomyces coelicolor or the close relative Corynebacterium glutamicum. Therefore, we screened the published genome sequences of Mycobacterium smegmatis, Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium avium ssp. paratuberculosis and Mycobacterium leprae for genes encoding proteins for uptake of nitrogen sources, nitrogen assimilation and nitrogen control systems, resulting in a detailed comparative genomic analysis of nitrogen metabolism-related genes for all completely sequenced members of the genus. Transporters for ammonium, nitrate, and urea could be identified, as well as enzymes crucial for assimilation of these nitrogen sources, i.e. glutamine synthetase, glutamate dehydrogenase, glutamate synthase, nitrate reductase, nitrite reductase, and urease proteins. A reduction of genes encoding proteins for nitrogen transport and metabolism was observed for the pathogenic mycobacteria, especially for M. leprae. Signal transduction components identified for the different species include adenylyl- and uridylyltransferase and a P-II-type signal transduction protein. Exclusively for M. smegmatis, two homologs of putative nitrogen regulatory proteins were found, namely GlnR and AmtR, while in other mycobacteria, AmtR was absent and GlnR seems to be the nitrogen transcription regulator protein. Copyright (C) 2008 S. Karger AG, Basel