Degradation behavior and compatibility of micro, nanoHA/chitosan scaffolds with interconnected spherical macropores
Degradation behavior and compatibility of micro, nanoHA/chitosan scaffolds with interconnected spherical macropores
复制标题
具有互连球形大孔的微米纳米HA/壳聚糖支架的降解行为和相容性
DOI：
10.1016/j.ijbiomac.2017.03.175
复制
发表时间：
2017-10-01
影响因子：
8.2
通讯作者：
Li Hui
中科院分区：
文献类型：
--
作者：
Li Ruixin;Xu Cheng;Li Hui
羟基磷灰石/壳聚糖（HA/CS）复合材料在生物医学尤其是骨替代方面有着重要的应用。复合材料中无机粒子的形状和大小影响支架的力学性能、生物学性能和降解性能。本研究的目的是制备具有良好孔连通性的HA/CS支架，并分析其生物降解性能。制备了具有连通球形孔结构的微羟基磷灰石/壳聚糖（mHA/CS）和纳米羟基磷灰石/壳聚糖（nHA/CS）复合支架。利用显微CT分析了复合支架的结构参数。使用小鼠成骨细胞系MC 3 T3-E1测试并比较两种支架之间的细胞增殖和形态。研究复合材料在溶菌酶PBS溶液中的降解情况，测试降解率和还原糖含量，并通过扫描电子显微镜观察支架形态。结果表明，采用煅烧法和合成法制备的微米级HA和纳米级HA的红外光谱非常相似。EDAX成分分析表明microHA和nanoHA均为缺钙型HA。Micro-CT结果表明，支架具有相互连通的球形孔，结构参数相似。随着培养时间的延长，细胞存活率显著增加，但microHA/CS和nanoHA/CS支架之间无显著差异。随着降解时间的延长，支架结构逐渐被破坏，无机成分HA颗粒更加突出。(1)复合材料中无机粒子的形状和大小影响支架的力学性能、生物学性能和降解性能。本文制备了具有良好孔连通性的NanoHA/CS和microHA/CS支架，并对其生物降解性能进行了研究。(2)具有互连孔隙结构的支架为细胞增殖和维持其分化功能提供了必要的支持，其结构与新骨的结构和形态有关。以石蜡微球为致孔剂，采用模压成型和颗粒沥滤法制备了聚合物支架。(3)MicroHA/CS和nanoHA/CS复合支架材料是骨组织工程的潜在材料。(C)2017由Elsevier B. V.出版
Hydroxyapatite/Chitosan (HA/CS) composite have significant application in biomedical especially for bone replacement. Inorganic particle shape and size of composite affect the scaffold mechanical property, biological property, and degradation. The aim of this study was to fabricate HA/CS scaffold with good pore connectivity and analyze their biological, degradation properties. Microhydroxyapatite/chitosan (mHA/CS) and nanohydroxyapatite/chitosan (nHA/CS) composite scaffolds with interconnected spherical pore architectures were fabricated. Composite scaffolds structure parameters were analyzed using micro CT. Cell proliferation and morphology were tested and compared between two scaffolds using mouse osteoblastic cell line MC3T3-E1. To research the composite degradation in lysozyme PBS solution, degradation rate and reducing sugar content were tested, and scaffolds morphology were observed by SEM. The results showed that microHA and nanoHA were fabricated by being calcined and synthesis methods, and their infrared spectra are very similar. EDAX composition analysis demonstrated that both of microHA and nanoHA were calcium deficiency HA. Micro-CT results demonstrated the scaffolds had interconnected spherical pores, and the structure parameters were similar. Cell viabilities were significant increased with cultured time, but there were no significant difference between microHA/CS and nanoHA/CS scaffolds. Scaffold structure was gradually destroyed and inorganic composition HA particles are more prominent with degradation time.Significance:. (1) Inorganic particle shape and size of composite affect the scaffold mechanical property, biological property, and degradation. NanoHA/CS and microHA/CS scaffolds with good pore connectivity were fabricated and their biological, degradation properties were studied in this manuscript. (2) The scaffold with interconnected porosity construct provides the necessary support for cells to proliferate and maintain their differentiated function, and its architecture related to the structure and morphology of new bone. Polymer scaffolds were fabricated by the technique of compression molding and particulate leaching method, and paraffin microspheres were used as the porogen. (3) MicroHA/CS and nanoHA/CS composite scaffolds are potential materials for use in bone tissue engineering. (C) 2017 Published by Elsevier B.V.