HUMAN MAJOR HISTOCOMPATIBILITY COMPLEX CONTAINS A MINIMUM OF 19 GENES BETWEEN THE COMPLEMENT CLUSTER AND HLA-B
HUMAN MAJOR HISTOCOMPATIBILITY COMPLEX CONTAINS A MINIMUM OF 19 GENES BETWEEN THE COMPLEMENT CLUSTER AND HLA-B
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DOI：
10.1073/pnas.86.22.8955
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发表时间：
1989-11-01
影响因子：
11.1
通讯作者：
STROMINGER, JL
中科院分区：
文献类型：
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作者：
SPIES, T;BRESNAHAN, M;STROMINGER, JL
通过延长先前的435-kb染色体步移，从人主要组织相容性复合体（MHC）III类区域分离出600-kb的DNA片段。克隆的重叠互补序列的连续系列包含补体基因簇中的C2和HLA-B之间的整个555-kb间隔。已知该区域编码肿瘤坏死因子（TNF）α。和β，B144和主要热休克蛋白HSP 70。此外，一簇基因，BAT 1-BAT 5（HLA-B相关转录物）已经定位在TNF α基因附近。和TNF β。另外四个基因，通过分离相应的cDNA克隆与粘粒DNA探针进行鉴定。BAT 6-BAT 9的这些基因被定位在C2基因附近的120 kb区域内，该区域包括HSP 70基因对。这些结果，以及最近一项类似研究的补充数据，表明在MHC II类区域的C2-HLA-B间隔内存在至少19个基因。虽然这些基因的功能特性还不清楚，但它们可能参与免疫的某些方面。这一想法得到了重组小鼠中造血组织相容性基因座-1（Hh-1）在TNF α和TNF β之间的遗传作图的支持。和H-2S，其与人类中的补体基因簇同源。
A 600-kilobase (kb) DNA segment from the human major histocompatibility complex (MHC) class III region was isolated by extension of a previous 435-kb chromosome walk. The contiguous series of cloned overlapping cosmids contains the entire 555-kb interval between C2 in the complement gene cluster and HLA-B. This region is known to encode the tumor necrosis factors (TNFs) .alpha. and .beta., B144, and the major heat shock protein HSP70. Moreover, a cluster of genes, BAT1-BAT5 (HLA-B-associated transcripts) has been localized in the vicinity of the genes for TNF.alpha. and TNF.beta.. An additional four genes were identified by isolation of corresponding cDNA clones with cosmid DNA probes. These genes for BAT6-BAT9 were mapped near the gene for C2 within a 120-kb region that includes a HSP70 gene pair. These results, together with complementary data from a similar recent study, indicated the presence of a minimum of 19 genes within the C2-HLA-B interval of the MHC class II region. Although the functional properties of most of these genes are yet unknown, they may be involved in some aspects of immunity. This idea is supported by the genetic mapping of the hemopoietic histocompatibility locus-1 (Hh-1) in recombinant mice between TNF.alpha. and H-2S, which is homologous to the complement gene cluster in humans.