Fungicides induced triazole-resistance in Aspergillus fumigatus associated with mutations of TR46/Y121F/T289A and its appearance in agricultural fields
Fungicides induced triazole-resistance in Aspergillus fumigatus associated with mutations of TR46/Y121F/T289A and its appearance in agricultural fields
复制标题
杀菌剂诱导的烟曲霉三唑抗性与TR46/Y121F/T289A突变相关及其在农田中的出现
DOI：
10.1016/j.jhazmat.2016.12.013
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发表时间：
2017-03-15
影响因子：
13.6
通讯作者：
Yu, Yunlong
中科院分区：
文献类型：
--
作者：
Ren, Jingbei;Jin, Xiangxiang;Yu, Yunlong
烟曲霉的唑类耐药性是一个日益严重的公共卫生问题。这种阻力的来源已引起人们的广泛关注。本研究的目的是评估A.三唑类杀菌剂能诱导烟曲霉及其cyp 51 A相关突变，以及是否能诱导烟曲霉抗性菌株。烟曲霉存在于农田中。结果表明，A.农用三唑类（氟环唑、戊唑醇、丙环唑、己唑醇和叶菌唑）能诱导TR 46/Y121 F/T289 A、A284 T、G448 S和P222 Q突变的烟曲霉致病性。TR 46/Y121 F/T289 A是A.烟熏。从浙江省不同蔬菜和水果大棚中采集了144个土壤样品。其中，分离鉴定出2株伏立康唑耐药菌株（15号和44号）和1株伊曲康唑耐药菌株（51号），分别为TR 46/Y121 F/T289 A突变株和TR 34/L98 H/S297 T/F495 I突变株。这表明，A.烟曲霉已经分布在至少5.8%的温室中。这些发现可能意味着三唑类化合物的农业使用与A.烟曲霉对三唑类药物的敏感性及其cyp 51 A的相关突变。(C)2016爱思唯尔B. V.保留所有权利。
Azole resistance in Aspergillus fumigatus is a growing public health problem. The sources of this resistance have been gained much attention. The present study was conducted to assess if resistant strain of A. fumigatus and its associated mutations in cyp51A could be induced by triazole fungicides and whether the resistant strain of A. fumigatus exist in agricultural fields. The results indicated that the resistance in A. fumigatus with mutations of TR46/Y121F/T289A, A284T, G448S and P222Q could be induced by agricultural triazoles (epoxiconazole, tebuconazole, propiconazole, hexaconazole, and met-conazole). TR46/Y121F/T289A was the most common mutation in the induced resistant strain of A. fumigatus. A total of 144 soil samples were collected from different greenhouses for vegetables and fruits in Zhejiang, China. Among them, 2 voriconazole-resistant strains (No. 15 and 44) harboring the mutation of TR46/Y121F/T289A and 1 itraconazole-resistant strain (No. 51) harboring the mutation of TR34/L98H/S297T/F495I were isolated and identified. This implies that resistant strain of A. fumigatus has already distributed at least in 5.8% of the greenhouses. These findings might imply that there is a direct link between the agricultural use of triazoles and the appearance of the resistance in A. fumigatus to triazole medicals and its associated mutations in cyp51A. (C) 2016 Elsevier B.V. All rights reserved.