Glucose enhances tilapia against Edwardsiella tarda infection through metabolome reprogramming
Glucose enhances tilapia against Edwardsiella tarda infection through metabolome reprogramming
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葡萄糖通过代谢组重编程增强罗非鱼抵抗迟缓爱德华氏菌感染的能力
DOI：
10.1016/j.fsi.2016.12.010
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发表时间：
2017-02
影响因子：
4.7
通讯作者：
Peng Bo
中科院分区：
文献类型：
--
作者：
Zeng Zhao-hai;Du Chao-chao;Liu Shi-rao;Li Hui;Peng Xuan-xian;Peng Bo
我们最近的研究表明，罗非鱼在迟缓爱德华氏菌感染过程中的存活与其代谢物组学密切相关，存活的尼罗罗非鱼与死亡的尼罗罗非鱼具有不同的代谢物组学特征。葡萄糖是区分存活代谢组和死亡代谢组的关键代谢物。更重要的是，外源性给予葡萄糖的鱼大大提高了他们的生存感染，表明葡萄糖代谢组再利用的功能作用，称为重编程代谢组学。然而，重新编程的基本信息尚不可用。本文采用基于GC/MS的代谢组学方法研究了外源葡萄糖对尼罗罗非鱼抗迟缓肠杆菌感染能力的影响。结果表明，外源葡萄糖促进了硬脂酸和棕榈酸的生物合成，但减弱了TCA循环，从而增强了尼罗罗非鱼对细菌感染的抵抗力，这一点通过外源硬脂酸以Mx蛋白的方式增强尼罗罗非鱼对迟缓艾美耳球虫感染的免疫保护作用得到证实。这些结果表明，外源性葡萄糖重编程尼罗罗非鱼抗感染代谢组，其特征在于硬脂酸和棕榈酸的升高和TCA循环的衰减。因此，我们的研究结果提出了一个新的机制，葡萄糖促进不饱和脂肪酸的生物合成，以科普感染，从而突出了一个潜在的方式，提高鱼类免疫力的水产养殖。
We have recently reported that the survival of tilapia, Oreochromis niloticus,during Edwardsiella tarda infection is tightly associated with their metabolome, where the survivedO. niloticushas distinct metabolomic profile to dyingO. niloticus. Glucose is the key metabolite to distinguish the survival- and dying-metabolome. More importantly, exogenous administration of glucose to the fish greatly enhances their survival for the infection, indicating the functional roles of glucose in metabolome repurposing, known as reprogramming metabolomics. However, the underlying information for the reprogramming is not yet available. Here, GC/MS based metabolomics is used to understand the mechanisms by which how exogenous glucose elevatesO. niloticus,anti-infectious ability toE. tarda. Results showed that exogenous glucose promotes stearic acid and palmitic acid biosynthesis but attenuates TCA cycle to potentiateO. niloticusagainst bacterial infection, which is confirmed by the fact that exogenous stearic acid increases immune protection inO. niloticusagainstE. tardainfection in a manner of Mx protein. These results indicate that exogenous glucose reprogramsO. niloticusanti-infective metabolome that characterizes elevation of stearic acid and palmitic acid and attenuation of the TCA cycle. Therefore, our results proposed a novel mechanism that glucose promotes unsaturated fatty acid biosynthesis to cope with infection, thereby highlighting a potential way of enhancing fish immunity in aquaculture.
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影响因子：
4.4
作者：
Xian-liang Zhao;Chang-wen Wu;Xuan-xian Peng;Hui Li
通讯作者：
Xian-liang Zhao;Chang-wen Wu;Xuan-xian Peng;Hui Li
影响因子：
3.9
作者：
Haller, Otto;Staeheli, Peter;Kochs, Georg
通讯作者：
Kochs, Georg
迟缓爱德华氏菌通过旁路途径阻止补体激活，从而逃避血清杀灭。
影响因子：
4.7
作者：
Li, Mo-fei;Sun, Li;Li, Jun
通讯作者：
Li, Jun
功能代谢组学：从生物标志物发现到代谢组重编程
影响因子：
21.1
作者：
Peng B;Li H;Peng XX
通讯作者：
Peng XX
影响因子：
5.5
作者：
Gao, Yuan;Wu, Haizhen;Zhang, Yuanxing
通讯作者：
Zhang, Yuanxing