ATPase and MHC class II molecules co-expression in Rana pipiens dendritic cells
ATPase and MHC class II molecules co-expression in Rana pipiens dendritic cells
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DOI：
10.1016/s0145-305x(99)00031-2
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发表时间：
1999-09-01
影响因子：
2.9
通讯作者：
Rondán-Zarate, A
中科院分区：
文献类型：
--
作者：
Castell-Rodríguez, AE;Hernández-Peñaloza, A;Rondán-Zarate, A
哺乳动物朗格汉斯细胞是位于不同上皮细胞的抗原呈递细胞。这些细胞具有特有的超微结构模式，具有质膜 ATP 酶活性并组成型表达主要组织相容性复合体的 II 类分子。在两栖动物表皮中也发现了形态与朗格汉斯细胞相似的 ATP 酶阳性树突状细胞。为了证明两栖动物表皮的 ATP 酶阳性树突状细胞表达 II 类分子并且存在于其他复层上皮中，进行了组织化学和免疫组织化学以及超微结构分析。在表皮、瞬膜和角膜中观察到ATP酶阳性树突状细胞和II类阳性树突状细胞。在表皮中，ATPase 阳性树突细胞的数量为 656 +/- 186/mm(2)，而 II 类阳性树突细胞的数量为 119 +/- 45/mm(2)。一些 ATPase 阳性树突状细胞表现出 II 类分子的共表达。这些结果表明两栖动物中存在树突状细胞亚群，这在哺乳动物中得到了清楚的证明。 (C) 1999 年由爱思唯尔科学有限公司出版。保留所有权利。
Mammalian Langerhans cells are antigen-presenting cells located in different epithelia. These cells have a characteristic ultrastructural pattern, present a plasmatic membrane ATPase activity and constitutively express class II molecules of the major histocompatibility complex. ATPase-positive dendritic cells that are morphologically similar to Langerhans cells have also been found in amphibian epidermis. In order to demonstrate that ATPase-positive dendritic cells of amphibian epidermis express class II molecules and are present in other stratified epithelia, histochemical and immunohistochemical as well as ultrastructural analysis were performed. ATPase-positive dendritic cells and class II-positive dendritic cells were observed in epidermis, nictitant membrane and cornea. In epidermis the number of ATPase-positive dendritic cells was 656 +/- 186/mm(2) while class II-positive dendritic cells was 119 +/- 45/mm(2). Some ATPase-positive dendritic cells showed co-expression of class II molecules. These results suggest the existence of dendritic cell subsets in amphibians as is clearly demonstrated in mammals. (C) 1999 Published by Elsevier Science Ltd. All rights reserved.