Opsonic potential, protective capacity, and sequence conservation of the Treponema pallidum subspecies pallidum Tp92
Opsonic potential, protective capacity, and sequence conservation of the Treponema pallidum subspecies pallidum Tp92
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DOI：
10.1086/315399
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发表时间：
2000-04-01
影响因子：
6.4
通讯作者：
Van Voorhis, WC
中科院分区：
文献类型：
--
作者：
Cameron, CE;Lukehart, SA;Van Voorhis, WC
通过一个差异筛选技术，92 kDa的抗原，指定TP 92，从梅毒螺旋体梅毒亚种被确定。该蛋白质在序列上与流感嗜血杆菌的保护性表面抗原D15和多杀性巴氏杆菌的Oma 87相似。氨基酸序列分析揭示了一个可切割的N端信号序列，并预测了Tp 92的外膜定位。梅毒螺旋体由兔巨噬细胞，和抗Tp 92反应性是不存在的洗涤密螺旋体制剂假定缺乏外膜。Tp 92氨基酸序列在代表4种致病性密螺旋体的11株菌株中具有95.5%-100%的保守性，重组Tp 92免疫家兔可部分保护其免受随后的T.苍白球挑战这些结果表明，Tp 92是一个不变的，免疫保护性抗原，可能存在于T。pallidum和可能代表一个潜在的候选疫苗梅毒。
By means of a differential screening technique, a 92-kDa antigen, designated Tp92, was identified from Treponema pallidum subspecies pallidum. This protein is similar in sequence to the protective surface antigens D15 from Haemophilus influenzae and Oma87 from Pasteurella multocida. Amino acid sequence analyses revealed a cleavable N-terminal signal sequence and predicted the outer membrane location for Tp92, In support of this, antiserum raised against recombinant Tp92 promotes opsonization and phagocytosis of T. pallidum by rabbit macrophages, and anti-Tp92 reactivity is absent from washed treponemal preparations presumed to be lacking outer membranes. The Tp92 amino acid sequence is 95.5%-100% conserved among 11 strains representing 4 pathogenic treponemes, and immunization with recombinant Tp92 partially protected rabbits from subsequent T. pallidum challenge. These results demonstrate that Tp92 is an invariant, immunoprotective antigen that may be present on the surface of T. pallidum and may represent a potential vaccine candidate for syphilis.