In vivo corneal endothelium imaging using ultrahigh resolution OCT.
In vivo corneal endothelium imaging using ultrahigh resolution OCT.
复制标题
使用超高分辨率 OCT 进行体内角膜内皮成像。
DOI：
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发表时间：
2019
影响因子：
3.4
通讯作者：
L. Schmetterer
中科院分区：
文献类型：
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作者：
X. Yao;Kavya Devarajan;R. Werkmeister;V. A. Dos Santos;M. Ang;A. Kuo;D. Wong;Jacqueline Chua;Bingyao Tan;V. Barathi;L. Schmetterer
我们研究了光学相干断层扫描（OCT）系统分辨率对猴眼高质量活体面角膜内皮细胞图像的影响，以便对细胞密度进行定量分析。我们通过使用不同的物镜来改变超高分辨率（UHR） OCT系统（以850 nm为中心）的横向分辨率，并通过开窗源光谱来改变轴向分辨率。通过抑制动物的运动，我们能够首次使用UHR OCT获得高质量的活体角膜内皮细胞图，横向分辨率为3.1µm。增加横向分辨率并不能提高成像质量，反而会导致视野变小，轴向分辨率对角膜内皮细胞的可视化影响不大。对角膜内皮细胞的活体OCT图像进行细胞密度定量分析，结果与先前报道的数据一致。我们的研究可能为设计高质量的活体角膜内皮细胞成像OCT系统提供实用的指导。
We investigate the influence of optical coherence tomography (OCT) system resolution on high-quality in vivo en face corneal endothelial cell images of the monkey eye, to allow for quantitative analysis of cell density. We vary the lateral resolution of the ultrahigh resolution (UHR) OCT system (centered at 850 nm) by using different objectives, and the axial resolution by windowing the source spectrum. By suppressing the motion of the animal, we are able to obtain a high-quality en face corneal endothelial cell map in vivo using UHR OCT for the first time with a lateral resolution of 3.1 µm. Increasing lateral resolution did not result in a better image quality but a smaller field of view (FOV), and the axial resolution had little impact on the visualization of corneal endothelial cells. Quantitative analysis of cell density was performed on in vivo en face OCT images of corneal endothelial cells, and the results are in agreement with previously reported data. Our study may offer a practical guideline for designing OCT systems that allow for in vivo corneal endothelial cell imaging with high quality.
使用展宽脉冲锁模激光器在 19 MHz 下进行相位稳定多普勒 OCT。
影响因子：
3.4
作者：
Tozburun,Serhat;Blatter,Cedric;Siddiqui,Meena;Meijer,EelcoFJ;Vakoc,BenjaminJ
通讯作者：
Vakoc,BenjaminJ