Ran is required before metaphase for spindle assembly and chromosome alignment and after metaphase for chromosome segregation and spindle midbody organization
Ran is required before metaphase for spindle assembly and chromosome alignment and after metaphase for chromosome segregation and spindle midbody organization
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DOI：
10.1091/mbc.e05-10-0991
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发表时间：
2006-04-01
影响因子：
3.3
通讯作者：
Wilde, A
中科院分区：
文献类型：
--
作者：
Silverman-Gavrila, RV;Wilde, A
Ran通路已被证明在纺锤体组装中起作用。然而，Ran通路在体内有丝分裂中的作用程度尚不清楚。我们报道Ran通路的扰动破坏了果蝇合胞胚胎中有丝分裂的多个步骤，并揭示了由Ran调节的新的有丝分裂过程。在有丝分裂开始时，Ran途径是中心体有核微管的产生、组织和靶向到染色体所必需的。然而，Ran的作用并不局限于微管组织，因为在中期板上染色体的排列也需要Ran。此外，Ran通路是中期后事件所必需的，包括染色体分离和微管中体的组装。Ran途径通过促进激酶Aurora A和激酶KLP61F和KLP3A对纺锤体的正确靶向，在一定程度上介导了这些有丝分裂事件。
The Ran pathway has been shown to have a role in spindle assembly. However, the extent of the role of the Ran pathway in mitosis in vivo is unclear. We report that perturbation of the Ran pathway disrupted multiple steps of mitosis in syncytial Drosophila embryos and uncovered new mitotic processes that are regulated by Ran. During the onset of mitosis, the Ran pathway is required for the production, organization, and targeting of centrosomally nucleated microtubules to chromosomes. However, the role of Ran is not restricted to microtubule organization, because Ran is also required for the alignment of chromosomes at the metaphase plate. In addition, the Ran pathway is required for postmetaphase events, including chromosome segregation and the assembly of the microtubule midbody. The Ran pathway mediates these mitotic events, in part, by facilitating the correct targeting of the kinase Aurora A and the kinesins KLP61F and KLP3A to spindles.