Erratum: The rise and fall of methanotrophy following a deepwater oil-well blowout
Erratum: The rise and fall of methanotrophy following a deepwater oil-well blowout
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勘误表：深水油井井喷后甲烷氧化菌的兴衰
DOI：
10.1038/ngeo2447
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发表时间：
2015
影响因子：
18.3
通讯作者：
Chanton, J. P.
中科院分区：
文献类型：
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作者：
Crespo-Medina, M.;Meile, C. D.;Hunter, K. S.;Diercks, A-R.;Asper, V. L.;Orphan, V. J.;Tavormina, P. L.;Nigro, L. M.;Battles, J. J.;Chanton, J. P.
深水地平线油井井喷的特殊情况需要采用标准操作程序。结果，我们在研究中采集的 1,128 个样本中，有 248 个样本没有在样本采集后 12-24 小时内启动甲烷氧化率测定（补充表 1），因为一些游轮缺乏放射性同位素隔离车，样本必须在游轮结束后运往佐治亚大学进行处理。具体而言，在 RV Pelican、RV Walton Smith、RV Arctic Sunrise 和 RV Nancy Foster 巡游中，样品在开始速率测定之前储存长达 29 天。补充表 1 中给出了这些航行的样本存储时间。为了评估存储和运输中产生的潜在问题，我们测量了船上和存储后的溶解氧和营养物质的浓度，并目视检查瓶子中是否有气泡以及微生物量的可见变化（例如，浊度、聚集）。在船上，样品无顶部空间地储存在气密瓶中，置于温度设定为 4℃的冰箱中。至少每四个小时检查一次冰箱的温度以确保稳定性。我们准备将样品运往雅典（美国佐治亚州），将其放在一层聚苯乙烯泡沫塑料花生（约 2.5 厘米厚）上，再覆盖一层蓝色冰袋。同样，蓝色冰袋排列在每个冷却器的侧面，并用纸板与样品瓶隔开。将一块纸板放置在样品顶部，然后将冰袋放置在纸板顶部。运输过程中没有出现变暖现象，样品在抵达雅典之前一直保持在深海温度（3-4°C）。
The special circumstances of the Deepwater Horizon oil-well blowout required adaptation of standard operating procedures. As a result, methane oxidation rate assays on 248 of the 1,128 samples we took in our study were not initiated within 12–24 hours of sample collection (Supplementary Table 1), because some cruises lacked a radioisotope isolation van and samples had to be shipped to the University of Georgia for processing after the cruise ended. Specifically, on the RV Pelican, RV Walton Smith, RV Arctic Sunrise, and the RV Nancy Foster cruises, samples were stored for up to 29 days before initiation of rate assays. Sample storage times for these cruises are given in Supplementary Table 1.To evaluate potential issues arising from storage and shipping, we measured the concentration of dissolved oxygen and nutrients on the ship and after storage, and bottles were visually inspected for gas bubbles, and visible changes (for example, turbidity, aggregations) in microbial biomass. On board the ship, samples were stored without a headspace in a gas-tight bottle in a refrigerator set at 4 C. The temperature of the refrigerator was checked at least every four hours to assure stability. We prepared samples for shipment to Athens (Georgia, USA) by placing them on a layer of Styrofoam peanuts (∼ 2.5 cm thick), which overlaid a layer of blue ice packs. Similarly, blue ice packs lined the sides of each cooler and were separated from the sample bottles by cardboard. A piece of cardboard was placed on top of the samples, and an ice pack was then placed on top of the cardboard. No warming occurred during shipment and samples remained at deep-sea temperatures (3–4 C) until arrival in Athens.