Identification of genes differentially expressed in a newly isolated human metastasizing esophageal cancer cell line, T.Tn-AT1, by cDNA microarray
Identification of genes differentially expressed in a newly isolated human metastasizing esophageal cancer cell line, T.Tn-AT1, by cDNA microarray
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DOI：
10.1111/j.1349-7006.2003.tb01505.x
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发表时间：
2003-08-01
期刊：
影响因子：
5.7
通讯作者：
Fujimori, T
中科院分区：
文献类型：
--
作者：
Kawamata, H;Furihata, T;Fujimori, T
我们通过体外筛选和裸鼠原位接种模型，从亲本非转移细胞系T.Tn中分离出转移性人食管鳞状细胞癌（SCC）细胞系T. Tn-AT 1。利用cDNA微阵列技术比较了T. Tn-AT 1和T.Tn中9206个基因的表达谱。Tn和T. Tn-AT 1的基因表达谱非常相似，只有34个基因表现出3倍以上的差异表达。在这34个基因中，有29个基因在T. Tn-AT 1细胞中表达下调，只有5个基因表达上调。随后，我们通过逆转录-聚合酶链反应证实了34个基因中的14个在T.Tn和T. Tn-AT 1细胞中的表达水平。Tn-AT 1细胞中有8个基因（KAL 1、HPGD、NDN、REG 1A、CXCR 4、SPOCK、DIAPH 2和AIF 1）表达下调，1个基因（VNN 2）表达上调。这9个基因编码与转移过程相关的蛋白质，如粘附、迁移、炎症、增殖和分化。因此，这些基因可能调控食管鳞癌的转移，并可能成为食管鳞癌淋巴结转移的预测指标。
We isolated a metastasizing human esophageal squamous cell carcinoma (SCC) cell line, T.Tn-AT1, from a parental non-metastasizing cell line, T.Tn, by in vitro selection and by use of a nude mouse orthotopic inoculation model. Then, we compared the expression profiles of 9206 genes in T.Tn-AT1 and T.Tn by cDNA microarray analysis. The gene expression profiles of T.Tn and T.Tn-AT1 were very similar, and only 34 genes showed more than 3-fold differential expression. Among the 34 genes, 29 genes were down-regulated and only 5 genes were up-regulated in T.Tn-AT1 cells. Subsequently, we confirmed the expression levels of 14 of the 34 genes in T.Tn and T.Tn-AT1 cells by means of reverse transcription-polymerase chain reaction. The expression of 8 genes (KAL1, HPGD, NDN, REG1A, CXCR4, SPOCK, DIAPH2 and AIF1) was down-regulated and that of one gene (VNN2) was up-regulated in T.Tn-AT1 cells. These 9 genes encoded proteins associated with metastatic processes, such as adhesion, migration, inflammation, proliferation, and differentiation. Thus, these genes might regulate the metastasis of esophageal SCC, and could be predictive markers for lymph node metastasis of esophageal SCC.