Autophagic response of intestinal epithelial cells exposed to polystyrene nanoplastics
Autophagic response of intestinal epithelial cells exposed to polystyrene nanoplastics
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DOI：
10.1002/tox.23678
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发表时间：
2022-09
影响因子：
4.5
通讯作者：
Xin Xu;Yudong Feng;Chenjie Han;Zhengrong Yao;Yaxin Liu;C. Luo;J. Sheng
中科院分区：
文献类型：
--
作者：
Xin Xu;Yudong Feng;Chenjie Han;Zhengrong Yao;Yaxin Liu;C. Luo;J. Sheng
越来越多的证据表明，聚苯乙烯纳米塑料（PS-NPs）在胃肠道中的生物累积对健康有负面影响。到目前为止，关于PS-NP对肠上皮屏障的潜在危害的信息很少。在这项研究中，我们采用细胞和动物模型来研究PS-NPs对肠上皮的不良影响及其潜在机制。我们发现PS-NPs以时间和浓度依赖性方式影响肠上皮细胞的生长和存活。PS-NP在细胞质中积累，导致自噬通量受损并诱导自噬反应。这种反应也在体内得到证实。我们的研究结果为PS-NPs的内化以及由此产生的肠上皮细胞自噬反应提供了新的见解。
Growing evidence demonstrates that the bioaccumulation of polystyrene nanoplastics (PS‐NPs) in the gastrointestinal tract has negative effects on health. Until now, little information has been available regarding the potential hazards of PS‐NPs to intestinal epithelial barriers. In this study, we employed cellular and animal models to investigate the adverse effects of PS‐NPs on intestinal epithelium and the underlying mechanism. We found that PS‐NPs affected the growth and survival of intestinal epithelial cells in a time‐ and concentration‐dependent manner. PS‐NPs accumulated in the cytoplasm, resulting in an impaired autophagic flux and inducing an autophagic response. This response was also confirmed in vivo. Our results provide new insights into the internalization of PS‐NPs and the resultant autophagy response in intestinal epithelial cells.