The COG complex interacts with multiple Golgi SNAREs and enhances fusogenic assembly of SNARE complexes
The COG complex interacts with multiple Golgi SNAREs and enhances fusogenic assembly of SNARE complexes
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DOI：
10.1242/jcs.122101
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发表时间：
2013-03-15
影响因子：
4
通讯作者：
Lev, Sima
中科院分区：
文献类型：
--
作者：
Laufman, Orly;Hong, WanJin;Lev, Sima
多亚基拴系复合物（MTCs）正调控囊泡融合的机制尚不清楚。在这项研究中，我们提供的证据表明，MTC COG增强融合高尔基陷阱复合物的组装，并伴随着防止非融合tSNARE相互作用。这种能力可能是由COG亚基和特定的高尔基体SNARE和SM（Sec 1/Munc 18）蛋白的多种直接相互作用介导的。通过使用系统的免疫共沉淀分析，我们确定了7个新的COG亚基和哺乳动物细胞中的高尔基体融合机制的组件之间的相互作用。我们的研究表明，这些多价的相互作用是至关重要的融合SNARE复合物的装配上的高尔基体，从而促进内体到trans-Golgi网络（TGN）和内高尔基体逆行运输，并协调这些运输路线。
Multisubunit tethering complexes (MTCs) positively regulate vesicular fusion by as yet unclear mechanism. In this study we provide evidence that the MTC COG enhances the assembly of fusogenic Golgi SNARE complexes and concomitantly prevents nonfusogenic tSNARE interactions. This capability is possibly mediated by multiple direct interactions of COG subunits and specific Golgi SNAREs and SM (Sec1/Munc18) proteins. By using a systematic co-immunoprecipitation analysis, we identified seven new interactions between the COG subunits and components of the Golgi fusion machinery in mammalian cells. Our studies suggest that these multivalent interactions are critical for the assembly of fusogenic SNARE complexes on the Golgi apparatus and consequently for facilitating endosome-to-trans-Golgi network (TGN) and intra-Golgi retrograde transport, and also for coordinating these transport routes.