Ultra-high performance, solid-state, autoradiographic image digitization and analysis system.
Ultra-high performance, solid-state, autoradiographic image digitization and analysis system.
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超高性能、固态、放射自显影图像数字化和分析系统。
DOI：
10.1007/bf01001046
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发表时间：
1990
影响因子：
3.6
通讯作者：
Rumley,S
中科院分区：
文献类型：
--
作者：
Lear,JL;Pratt,JP;Ackermann,RF;Plotnick,J;Rumley,S
我们开发了一种基于Macintosh II的电荷耦合器件（CCD）图像数字化和分析系统，用于放射自显影图像数据的高速、高分辨率量化。将具有3500个元件的线性CCD阵列连接到精密驱动组件并安装在高均匀性透镜后面。驱动组件用于垂直于其轴扫描阵列，以便整个20 × 25 cm的放射自显影图像包含胶片可以在不到30秒的时间内数字化为256个灰度级，分辨率为50 μm。扫描仪通过专门构造的NuBus电路板与Macintosh II计算机连接，并开发了用于放射自显影数据分析的软件。通过多次扫描单个胶片，然后测量不同扫描之间的数字数据的可变性来评估该系统。图像数据被发现几乎是无噪声的。变异系数平均小于1%，该值显著超过了高速、低分辨率摄像机（VC）系统和低速、高分辨率转鼓密度计（RDD）的精度。因此，CCD扫描仪-Macintosh计算机分析系统提供了优于VC系统的优点，即能够扫描包含许多放射自显影图的整个胶片，但速度和精度比RDD扫描仪高得多。
We developed a Macintosh II-based, charge-coupled device (CCD), image digitization and analysis system for high-speed, high-resolution quantification of autoradiographic image data. A linear CCD array with 3500 elements was attached to a precision drive assembly and mounted behind a high-uniformity lens. The drive assembly was used to sweep the array perpendicularly to its axis so that an entire 20 × 25-cm autoradiographic image-containing film could be digitized into 256 gray levels at 50-μm resolution in less than 30 sec. The scanner was interfaced to a Macintosh II computer through a specially constructed NuBus circuit board and software was developed for autoradiographic data analysis. The system was evaluated by scanning individual films multiple times, then measuring the variability of the digital data between the different scans. Image data were found to be virtually noise free. The coefficient of variation averaged less then 1%, a value significantly exceeding the accuracy of both high-speed, low-resolution, video camera (VC) systems and low-speed, high-resolution, rotating drum densitometers (RDD). Thus, the CCD scanner-Macintosh computer analysis system offers the advantage over VC systems of the ability to digitize entire films containing many autoradiograms, but with much greater speed and accuracy than achievable with RDD scanners.