Effect of adhesion or collagen molecules on cell attachment, insulin secretion, and glucose responsiveness in the cultured adult porcine endocrine pancreas: A preliminary study
Effect of adhesion or collagen molecules on cell attachment, insulin secretion, and glucose responsiveness in the cultured adult porcine endocrine pancreas: A preliminary study
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DOI：
10.3727/000000003108746867
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发表时间：
2003-01-01
影响因子：
3.3
通讯作者：
Ohgawara, H
中科院分区：
文献类型：
--
作者：
Edamura, K;Nasu, K;Ohgawara, H
在体外长期培养的成年猪胰腺内分泌(PE)细胞中，研究了黏附分子和胶原分子对细胞贴壁、胰岛素分泌和葡萄糖反应性的影响。使用了六种不同类型的分子-层粘连蛋白、纤维连接蛋白、多L赖氨酸、I型胶原、明胶和Matrigel。每皿约2.0×10(5)个细胞，每种分子类型培养4周。层粘连蛋白组在培养4周时胰岛素积累量明显高于对照组，葡萄糖刺激的胰岛素分泌和胰岛素阳性率也高于对照组。Matrigel组可见胰岛样细胞团形成，但胰岛素积聚在培养3~4周后迅速下降。培养至第4周，纤维连接蛋白组可见大量PE细胞紧密贴壁、铺展，但其功能并不优于对照组。PLL组和明胶组PE细胞功能与对照组无明显差异。I型胶原组胰岛素分泌低于其他组。本研究结果提示，层粘连蛋白是最适合PE细胞长期培养保存的细胞外基质。
The effect of either adhesion or collagen molecules on cell attachment, insulin secretion, and glucose responsiveness was investigated in adult porcine pancreatic endocrine (PE) cells that were cultured for a longer term in vitro. Six different types of molecules-laminin, fibronectin, poly-L-lysine (PLL), type I collagen, gelatin, and Matrigel-were used. Approximately 2.0 x 10(5) cells per dish of each molecule type were cultured for 4 weeks. In the laminin group, the insulin accumulation was maintained at a significantly higher level than in the control group at 4 weeks of culture, and glucose-stimulated insulin secretion and the insulin-positive rate were also higher than in the control group. In the Matrigel group, islet-like cell clusters were formed, but insulin accumulation rapidly decreased at 3-4 weeks of culture. A large number of PE cells attached tightly and spread in the fibronectin group until the fourth week of culture, but their function was not better than those in the control group. In the PLL and gelatin groups, the PE cell function was not significantly different from that of the control group. In the type I collagen group, insulin secretion was inferior to that of the other groups. The results of this study suggest that laminin is the most suitable extracellular matrix for the long-term culture preservation of PE cells.