Evaluation of swabs from 15 commercially available oral fluid sample collection devices for the analysis of commonly abused substances: doping agents and drugs of abuse
Evaluation of swabs from 15 commercially available oral fluid sample collection devices for the analysis of commonly abused substances: doping agents and drugs of abuse
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DOI：
10.1039/d0an01379j
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发表时间：
2020-11-21
期刊：
影响因子：
4.2
通讯作者：
Gorynski, Krzysztof
中科院分区：
文献类型：
--
作者：
Sobczak, Lukasz;Gorynski, Krzysztof
口腔液体检测正在稳步建立其作为一个有价值的补充或替代血浆和尿液分析在日常实验室实践中的地位。然而，样本收集过程在取得有代表性的结果方面的重要意义并不总是被对其知情选择的重视所掩盖。到目前为止，已经发表了一些对市售样本采集设备的评估，目前的工作旨在通过对15种不同设备的拭子进行评估来填补这一空白，以分析49种流行药物。使用来自样本采集装置的拭子采集药物强化混合物。然后，拭子回收的样品进行仪器分析与高效液相色谱-串联质谱法。认为80-120%范围内的结果对分析物浓度无显著影响（因此符合要求），在所有结果的44.1%中观察到。在评价的15个拭子中，7个拭子提供了超过一半的研究物质的上述范围内的结果。还研究了源自拭子材料的基质效应的可能性。选择适当的口腔液样本采集方法在分析方法的成功中起着关键作用，本研究中观察到的分析物浓度之间的巨大差异很好地说明了这一事实。如果更加重视改进和更好地选择用于口腔液采集的合适溶液，也许可以省去改进已经使用的样品制备和分析方法的繁琐劳动。
Oral fluid testing is steadily building its position as a valuable complement or alternative to plasma and urine analyses in everyday laboratory practice. However, the great significance of the sample collection process in the attainment of representative results is not always paralleled by the attention given to its informed selection. Few evaluations of commercially available sample collection devices have been published until now, and the current work intends to fill this gap by presenting an evaluation of swabs from 15 different devices for the analysis of 49 popular drugs. Swabs, derived from sample collection devices, were used to collect a drug-fortified mixture. Then, swab-retrieved samples were subjected to instrumental analysis with the high-performance liquid chromatography coupled with tandem mass spectrometry method. Results within the 80-120% range were considered to have no significant impact on analyte concentration (thus satisfactory) and were observed in 44.1% of all results. Out of the 15 evaluated swabs, 7 provided results in the aforementioned range for more than half of the substances under study. The possibility of matrix effects originating from swab materials was also investigated. The selection of an appropriate oral fluid sample collection method plays a critical role in the success of the analytical procedure, a fact that is well-illustrated by the tremendous differences between analyte concentrations observed in this research. Perhaps, the tedious labour of improving sample preparation and analysis methods already in-use could be spared if only greater emphasis were to be put on the improvement and better selection of suitable solutions for oral fluid collection.