Taenia asiatica and Taenia saginata:: Genetic divergence estimated from their mitochondrial genomes
Taenia asiatica and Taenia saginata:: Genetic divergence estimated from their mitochondrial genomes
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DOI：
10.1016/j.exppara.2005.11.018
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发表时间：
2006-05-01
影响因子：
2.1
通讯作者：
Eom, K. S.
中科院分区：
文献类型：
--
作者：
Jeon, H. K.;Eom, K. S.
通过线粒体基因组的定位和cox 1和cob基因的测序，我们对亚洲带绦虫和牛带绦虫进行了鉴别。整个线粒体基因组的亚洲和T saginata的长延伸PCR扩增和克隆，每个约14 kb的大小。然后，使用13种限制性内切酶构建了亚洲红豆杉和刀豆红豆杉线粒体基因组的限制性内切酶图谱。由此产生的限制模式使我们能够估计它们的遗传差异为4.8%。cox1基因的实际序列差异为4.5%，cob基因的实际序列差异为4.1%。这些结果支持了亚洲茶T asiatica作为一个独立的种从T saginata的指定。(c)2005年爱思唯尔公司All rights reserved.
We conducted a differential identification of Taenia asiatica and Taenia saginata, through the mapping of mitochondrial genomes and the sequencing of the cox1 and cob genes. The entire mitochondrial genomes of T asiatica and T saginata were amplified by long-extension PCR and cloned; each was approximately 14 kb in size. Restriction maps of T asiatica and T saginata mitochondrial genomes were then constructed using 13 restriction enzymes. The resulting restriction patterns enable us to estimate their genetic divergence at 4.8%. The actual sequence divergence was computed 4.5% from the cox1 gene, and 4.1% from the cob gene. These results support the designation of T asiatica as a separate species from T saginata. (c) 2005 Elsevier Inc. All rights reserved.