IDENTIFICATION OF A PROTEIN ENCODED BY THE VPU GENE OF HIV-1
IDENTIFICATION OF A PROTEIN ENCODED BY THE VPU GENE OF HIV-1
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DOI：
10.1038/334532a0
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发表时间：
1988-08-11
期刊：
影响因子：
64.8
通讯作者：
HASELTINE, WA
中科院分区：
文献类型：
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作者：
COHEN, EA;TERWILLIGER, EF;HASELTINE, WA
人类免疫缺陷病毒 1 (HIV-1) 是 AIDS1-3 的病原体。该病毒在培养的细胞中建立裂解性、潜伏性和非细胞病变的生产性感染4,5。病毒与宿主细胞相互作用的复杂性反映在病毒基因组的复杂组织中6-9。除了编码病毒颗粒衣壳和包膜蛋白的基因以及所有逆转录病毒共有的前病毒合成和整合所需的酶之外，已知 HIV-1 还编码至少四种调节病毒复制的附加蛋白：thetat、art、sorand 3'orf 蛋白，以及来自开放阅读框的功能未知的蛋白 R10-18。对多个 HIV 分离株基因组核酸序列的仔细检查提出了该病毒编码以前未检测到的额外蛋白质的可能性。在此，我们报道 HIV-1 编码第九种蛋白质，并且在 HIV-1 感染者的血清中检测到该蛋白质的抗体。这种蛋白质将 HIV-1 分离株与其他人类和猿猴免疫缺陷病毒（HIV-2 和 SIV）19-21 区分开来，后者不具备编码类似蛋白质的能力。
Human immunodeficiency virus 1 (HIV-1) is the aetiological agent of AIDS1–3. The virus establishes lytic, latent and non-cytopathic productive infection in cells in culture4,5. The complexity of virus‐host cell interaction is reflected in the complex organization of the viral genome6–9. In addition to the genes that encode the virion capsid and envelope proteins and the enzymes required for proviral synthesis and integration common to all retroviruses, HIV-1 is known to encode at least four additional proteins that regulate virus replication, thetat, art, sorand 3′orfproteins, as well as a protein of unknown function from the open reading frame called R10–18. Close examination of the nucleic acid sequences of the genomes of multiple HIV isolates raised the possibility that the virus encodes a previously undetected additional protein. Here we report that HIV-1 encodes a ninth protein and that antibodies to this protein are detected in the sera of people infected with HIV-1. This protein distinguishes HIV-1 isolates from the other human and simian immunodeficiency viruses (HIV-2 and SIV)19–21that do not have the capacity to encode a similar protein.