MMP-2 levels are elevated in the plasma of women who subsequently develop preeclampsia
MMP-2 levels are elevated in the plasma of women who subsequently develop preeclampsia
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DOI：
10.1081/prg-200059836
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发表时间：
2005-01-01
影响因子：
1.5
通讯作者：
Davidge, ST
中科院分区：
文献类型：
--
作者：
Myers, JE;Merchant, SJ;Davidge, ST
Objective.测定在临床疾病发作前注定要发展为先兆子痫的妇女血浆中基质金属蛋白酶（MMP）-2和MMP-9以及金属蛋白酶组织抑制剂（TIMP）-1和TIMP-2的水平。研究设计.从妊娠随后并发先兆子痫的妇女和正常妊娠妇女在22周和26周以及分娩或诊断时采集血浆样品。在等量蛋白加载后，分别使用酶谱法和Western印迹分析定量MMP-2和MMP-9以及TIMP-1和TIMP-2。结果子痫前期组在22周（p = 0.02）和诊断时（p = 0.003）血浆MMP-2水平显著升高，但在26周时无差异。子痫前期组在26周时TIMP-1水平显著降低（p = 0.0002），但TIMP-2水平无法定量。结论在所有三个妊娠时间点的MMP-2：TIMP-1比例失衡，发现在随后发展先兆子痫的患者。我们推测，增加净MMP-2活性可能有助于内皮功能障碍，是中央先兆子痫的病理生理。
Objective. To determine levels of matrix metalloproteinase (MMP)-2 and MMP-9, and the tissue inhibitors of metalloproteinases (TIMP)-1 and TIMP-2 in the plasma of women destined to develop preeclampsia prior to the onset of clinical disease. Study Design. Plasma samples were taken from women whose pregnancies were subsequently complicated by preeclampsia and from normal pregnant women at 22 and 26 weeks and at delivery or diagnosis. Following equal protein loading, MMP-2 and -9 and TIMP-1 and -2 were quantified using zymography and Western blot analysis, respectively. Results. Plasma MMP-2 levels were significantly elevated at 22 weeks (p = 0.02) and at diagnosis (p = 0.003) in the preeclampsia group, but there was no difference at 26 weeks. TIMP-1 levels were significantly reduced in the preeclampsia group at 26 weeks (p = 0.0002), but TIMP-2 levels were not quantifiable. Conclusion. At all three gestational time points an imbalance in the MMP-2:TIMP-1 ratio was found in patients who subsequently developed preeclampsia. We speculate that increased net MMP-2 activity may contribute to the endothelial dysfunction that is central to the pathophysiology of preeclampsia.