Subcompartmentalisation of proteins in the rhoptries correlates with ordered events of erythrocyte invasion by the blood stage malaria parasite.
Subcompartmentalisation of proteins in the rhoptries correlates with ordered events of erythrocyte invasion by the blood stage malaria parasite.
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DOI：
10.1371/journal.pone.0046160
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Baum J
中科院分区：
文献类型：
--
作者：
Zuccala ES;Gout AM;Dekiwadia C;Marapana DS;Angrisano F;Turnbull L;Riglar DT;Rogers KL;Whitchurch CB;Ralph SA;Speed TP;Baum J
顶复门寄生虫对宿主细胞的感染在这些专性胞内病原体的生命周期进展中起着重要作用。对于大多数物种，包括疟疾和弓形虫病的病原体，感染需要宿主细胞主动入侵，这取决于紧密连接的形成-寄生虫和宿主细胞之间的组织界面在进入过程中。然而，这种结构的形成并不是在所有顶复门或实际上所有寄生虫生命周期阶段中共享的。在这里，使用计算机集成基因组搜索和内源性基因标记策略，我们试图识别在连接依赖性入侵过程中特异性发挥作用的蛋白质，我们将一类蛋白质称为入侵素，以将其与在物种特异性宿主细胞识别中发挥作用的粘附素区分开来。标记的恶性疟原虫侵入蛋白质定位于血液期裂殖子的多个细胞区室的高清晰度成像。这包括几个本地化到不同的亚隔室内的棒状体。然而，虽然起源于相同的细胞器，但它们在入侵过程中具有非常不同的动力学。顶端寿司蛋白和棒状体颈蛋白2在连接后早期释放，而一种新的棒状体蛋白PFF 0645 c仅在入侵完成后释放。这支持了这样的想法，即组织内的分泌细胞器的蛋白质决定的顺序和目的地的蛋白质分泌，并提供了一个基于定位的分类策略，用于预测在apicomplexan寄生虫入侵的invasin功能。
Host cell infection by apicomplexan parasites plays an essential role in lifecycle progression for these obligate intracellular pathogens. For most species, including the etiological agents of malaria and toxoplasmosis, infection requires active host-cell invasion dependent on formation of a tight junction – the organising interface between parasite and host cell during entry. Formation of this structure is not, however, shared across all Apicomplexa or indeed all parasite lifecycle stages. Here, using an in silico integrative genomic search and endogenous gene-tagging strategy, we sought to characterise proteins that function specifically during junction-dependent invasion, a class of proteins we term invasins to distinguish them from adhesins that function in species specific host-cell recognition. High-definition imaging of tagged Plasmodium falciparum invasins localised proteins to multiple cellular compartments of the blood stage merozoite. This includes several that localise to distinct subcompartments within the rhoptries. While originating from the same organelle, however, each has very different dynamics during invasion. Apical Sushi Protein and Rhoptry Neck protein 2 release early, following the junction, whilst a novel rhoptry protein PFF0645c releases only after invasion is complete. This supports the idea that organisation of proteins within a secretory organelle determines the order and destination of protein secretion and provides a localisation-based classification strategy for predicting invasin function during apicomplexan parasite invasion.
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影响因子：
6.7
作者：
Collins, Christine R.;Withers-Martinez, Chrislaine;Hackett, Fiona;Blackman, Michael J.
通讯作者：
Blackman, Michael J.
影响因子：
3.7
作者：
Angrisano, Fiona;Riglar, David T.;Baum, Jake
通讯作者：
Baum, Jake
影响因子：
4.8
作者：
Bradley, PJ;Ward, C;Boothroyd, JC
通讯作者：
Boothroyd, JC
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影响因子：
1.9
作者：
Cao, Jun;Kaneko, Osamu;Torii, Motomi
通讯作者：
Torii, Motomi
影响因子：
64.8
作者：
DESAI, SA;KROGSTAD, DJ;MCCLESKEY, EW
通讯作者：
MCCLESKEY, EW