Overexpression of the steroidogenic acute regulatory protein increases the expression of ATP-binding cassette transporters in microvascular endothelial cells (bEnd.3)
Overexpression of the steroidogenic acute regulatory protein increases the expression of ATP-binding cassette transporters in microvascular endothelial cells (bEnd.3)
复制标题
类固醇生成急性调节蛋白的过度表达会增加微血管内皮细胞中 ATP 结合盒转运蛋白的表达 (bEnd.3)
DOI：
10.1631/jzus.b0900369
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发表时间：
2010-05-01
影响因子：
5.1
通讯作者：
Yin, Lian-hua
中科院分区：
文献类型：
--
作者：
Ning, Yan-xia;Ren, Shun-lin;Yin, Lian-hua
目的研究类固醇生成急性调节蛋白（星星）过表达对内皮细胞系（bEnd. 3）中三磷酸腺苷（ATP）结合盒转运体A1（ABCA 1）和ATP结合盒转运体G1（ABCG 1）水平的影响。采用荧光激活细胞分选仪（FACS）和荧光显微镜检测感染效率。采用实时荧光定量PCR和Western blot检测星星基因和蛋白的表达水平。结果星星基因在bEnd. 3细胞中成功过表达，并在转染后的细胞中表达了ABCA 1和ABCG 1的基因和蛋白。结论星星过表达可增加内皮细胞ABCA 1和ABCG 1的表达。
ObjectiveTo determine the effect of steroidogenic acute regulatory protein (StAR) overexpression on the levels of adenosine triphosphate (ATP)-binding cassette transporter A1 (ABCA1) and ATP-binding cassette transporter G1 (ABCG1) in an endothelial cell line (bEnd.3).MethodsThe StAR gene was induced in bEnd.3 cells with adenovirus infection. The infection efficiency was detected by fluorescence activated cell sorter (FACS) and fluorescence microscopy. The expressions of StAR gene and protein levels were detected by real-time polymerase chain reaction (PCR) and Western blot. The gene and protein levels of ABCA1 and ABCG1 were detected by real-time PCR and Western blot after StAR overexpression.ResultsThe result shows that StAR was successfully overexpressed in bEnd.3 cells by adenovirus infection. The mRNA and protein expressions of ABCA1 and ABCG1 were greatly increased by StAR overexpression in bEnd.3 cells.ConclusionOverexpression of StAR increases ABCA1 and ABCG1 expressions in endothelial cells.