Integrated cell manipulation system - CMOS/microfluidic hybrid
Integrated cell manipulation system - CMOS/microfluidic hybrid
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DOI：
10.1039/b700373k
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发表时间：
2007-01-01
期刊：
影响因子：
6.1
通讯作者：
Westervelt, Robert M.
中科院分区：
文献类型：
--
作者：
Lee, Hakho;Liu, Yong;Westervelt, Robert M.
使用CMOS/微流体混合系统的生物细胞的操纵被证明。混合系统从半导体代工厂制造的定制设计的CMOS（互补金属氧化物半导体）芯片开始。在CMOS芯片的顶部上后制造微流体通道以提供生物相容性环境。用磁珠标记的单个生物细胞的运动由CMOS芯片直接控制，该芯片使用微电磁体的芯片上阵列产生微观磁场图案。此外，CMOS芯片允许磁场的高速和可编程重新配置，大大提高了混合系统的操纵能力。扩展以前的工作，验证了混合系统的概念，本文报道了一组与生物细胞的操作实验，这进一步证实了混合方法的优势。为了增强系统的生物相容性，重新设计了微流体通道，并通过片上传感器监测装置的温度。结合微电子学和微流体学，CMOS/微流体混合系统提出了一种新的模式，在生物和生物医学应用中的细胞操作平台。
Manipulation of biological cells using a CMOS/microfluidic hybrid system is demonstrated. The hybrid system starts with a custom-designed CMOS ( complementary metal-oxide semiconductor) chip fabricated in a semiconductor foundry. A microfluidic channel is post-fabricated on top of the CMOS chip to provide biocompatible environments. The motion of individual biological cells that are tagged with magnetic beads is directly controlled by the CMOS chip that generates microscopic magnetic field patterns using an on-chip array of micro-electromagnets. Furthermore, the CMOS chip allows high-speed and programmable reconfiguration of the magnetic fields, substantially increasing the manipulation capability of the hybrid system. Extending from previous work that verified the concept of the hybrid system, this paper reports a set of manipulation experiments with biological cells, which further confirms the advantage of the hybrid approach. To enhance the biocompatibility of the system, the microfluidic channel is redesigned and the temperature of the device is monitored by on-chip sensors. Combining microelectronics and microfluidics, the CMOS/microfluidic hybrid system presents a new model for a cell manipulation platform in biological and biomedical applications.