In Vivo Measurement of Blood Velocity in Human Major Retinal Vessels Using the Laser Speckle Method
In Vivo Measurement of Blood Velocity in Human Major Retinal Vessels Using the Laser Speckle Method
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DOI：
10.1167/iovs.09-4422
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发表时间：
2011-01-01
影响因子：
4.4
通讯作者：
Araie, Makoto
中科院分区：
文献类型：
--
作者：
Nagahara, Miyuki;Tamaki, Yasuhiro;Araie, Makoto
目的.通过使用激光散斑方法并通过双向激光多普勒测速仪（LDV）验证结果，开发一种简单、无创的方法来确定人体视网膜血管（RV）的血液速度和流量。方法。平方模糊率（SBR），激光散斑图案的定量模糊指数，平行于移动物质的速度，从流过玻璃毛细管（RV类似物）的血液中获得，与管直径，背景反射率和吸收，流速，和SBR从流过下面的玻璃毛细管（脉络膜血管类似物）的血液中获得。从体内获得的SBR值构建诺模图以计算人RV中的血液速度。本文应用激光散斑法和双向LDV法测定了12只正常眼视网膜静脉的血流速度。在同一部位以1小时间隔进行两次测量。颞上级动脉的测量（n = 12;平均值+/- SD）为血流速度（V-平均值），41.7 +/- 4.2 mm/s;流量，13.0 +/- 3.2 μ L/min;直径，119.5 +/- 15.7 μ m，使用激光散斑仪完成一次测量的时间为65 +/- 18秒;和V-平均值，37.7 +/- 6.7 mm/s;流量，11.7 +/- 3.0 μ L/min;直径，111.1 +/- 16.6 μ m;和测量时间，112 +/- 25秒。两种方法获得的结果彼此相关（V均值，r = 0.59，P = 0.023;流量，r = 0.83，P = 0.005;直径，r = 0.56，P = 0.032）。V均值、血流量和直径测量的重现性系数分别为9.5% +/-2.5%、10.5% +/-3.2%和5.3% +/- 2.7%，前者为15.3% +/-4.2%、18.5% +/-4.1%和6.2% +/- 2.2%。结论。激光散斑方法在比LDV设备所需的更短的时间内准确地且可再现地确定人RV中的血液速度。（Invest Ophthalmol维斯科学。2011; 52：87-92）DOI：10.1167/iovs.09-4422
PURPOSE. To develop a simple, noninvasive method of determining blood velocity and flow through human retinal vessels (RVs), by using the laser speckle method and validating the results by bidirectional laser Doppler velocimetry (LDV).METHOD. The square blur rate (SBR), a quantitative blurring index of the laser speckle pattern that parallels the velocity of moving substances, obtained from blood flowing through glass capillary tubes (RV analogues), correlated with tube diameter, background reflectance and absorption, flow velocity, and the SBR obtained from blood flowing through underlying glass capillary tubes (choroidal vessel analogues). A nomogram was constructed to calculate the blood velocity in human RVs from the SBR values obtained in vivo. Blood velocities in RVs were determined in 12 normal eyes by using the laser speckle method and bidirectional LDV. Measurements were performed twice at the same site at 1-hour intervals.RESULTS. Measurements from a temporal superior artery (n = 12; mean +/- SD) were blood velocity (V-mean), 41.7 +/- 4.2 mm/s; flow, 13.0 +/- 3.2 mu L/min; and diameter, 119.5 +/- 15.7 mu m and time to complete one measurement, 65 +/- 18 seconds, with the laser speckle apparatus; and V-mean, 37.7 +/- 6.7 mm/s; flow, 11.7 +/- 3.0 mu L/min; diameter, 111.1 +/- 16.6 mu m; and measurement time, 112 +/- 25 seconds, with the bidirectional LDV apparatus. The results obtained by the two methods correlated with each other (V-mean, r = 0.59, P = 0.023; flow, r = 0.83, P = 0.005; and diameter, r = 0.56, P = 0.032). The coefficients of reproducibility for V mean, blood flow, and diameter measurement were 9.5% +/- 2.5%, 10.5% +/- 3.2%, and 5.3% +/- 2.7% for the former and 15.3% +/- 4.2%, 18.5% +/- 4.1%, and 6.2% +/- 2.2% for the latter, respectively.CONCLUSIONS. The laser speckle method accurately and reproducibly determines blood velocity in human RVs in less time than the LDV apparatus requires. (Invest Ophthalmol Vis Sci. 2011; 52: 87-92) DOI:10.1167/iovs.09-4422