Astragalus polysaccharides enhance immune responses of HBV DNA vaccination via promoting the dendritic cell maturation and suppressing Treg frequency in mice
Astragalus polysaccharides enhance immune responses of HBV DNA vaccination via promoting the dendritic cell maturation and suppressing Treg frequency in mice
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DOI：
10.1016/j.intimp.2012.09.006
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发表时间：
2012-12-01
影响因子：
5.6
通讯作者：
Zeng, Xianyin
中科院分区：
文献类型：
--
作者：
Du, Xiaogang;Zhao, Bing;Zeng, Xianyin
黄芪多糖是一种从中草药黄芪中提取的物质，具有很强的免疫调节作用。本研究将APS作为乙肝病毒DNA疫苗(PcDS2)的佐剂，研究其对小鼠免疫系统的影响。结果表明，APS作为佐剂可以提高T细胞的抗-HBs抗体水平和T细胞的增殖活性。APS还能诱导CD8(+)T细胞产生IL-4、IL-2和干扰素-γ，增强CD8(+)T细胞的干扰素-γ的表达。此外，APS还能诱导细胞毒性淋巴细胞(CTL)的活性增强。此外，APS还能刺激以MHC I/II、CD40、CD80和CD86上调为特征的树突状细胞(DC)成熟，并降低调节性T细胞(NTreg)的频率。综上所述，这些发现表明APS是一种有效的乙肝DNA疫苗佐剂，可以通过促进DC成熟和抑制Treg频率来增强HBVDNA疫苗的免疫应答。(C)2012爱思唯尔B.V.保留所有权利。
Astragalus polysaccharides (APS), an extract from a kind of Chinese traditional herb Astragalus membranaceus, was proved to have strong immunoregulatoiy properties. In this study, APS was employed as an adjuvant of Hepatitis B virus (HBV) DNA vaccine (pcDS2) and its' effects on immune system of mice were investigated. Our data demonstrated that APS as an adjuvant could increase the HBsAg-specific antibody level as well as the proliferating activity of T cells. APS also could induce CD4(+) T cells to produce IL-4, IL-2 and IFN-gamma and enhance IFN-gamma expression of CD8(+) T cells. Moreover, APS could induce the robust activity of the cytotoxic lymphocytes (CTL). Additionally, APS could stimulate the dendritic cells (DC) maturation which is characterized by up-regulation of MHC I/II, CD40, CD80 and CD86, and decreased the frequency of the regulatory T cells (nTreg). Collectively, these findings suggest that APS is a potent adjuvant for the hepatitis B DNA vaccine and can enhance the immune responses of HBV DNA vaccine via promoting DC maturation and inhibit the Treg frequency. (C) 2012 Elsevier B.V. All rights reserved.