Natural variation in Pristionchus pacificus insect pheromone attraction involves the protein kinase EGLA-4
Natural variation in Pristionchus pacificus insect pheromone attraction involves the protein kinase EGLA-4
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DOI：
10.1073/pnas.0708406105
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发表时间：
2008-06-03
影响因子：
11.1
通讯作者：
Sommer, Ralf J.
中科院分区：
文献类型：
--
作者：
Hong, Ray L.;Witte, Hanh;Sommer, Ralf J.
共同进化的地理镶嵌理论预测，不同当地物种的相互作用将塑造种群特征，但人们对调节这些相互作用的特异性所涉及的分子因素知之甚少。 Pristionchus 线虫与世界各地不同的圣甲虫有联系，其中 Pristionchus pacificus 主要从日本的东方甲虫中分离出来。特别是，的组成人口。太平洋对虾为研究多种专门相互作用以及在遗传水平上影响种群性状的机制提供了难得的机会。我们使用靶向基因渗入系、外源 cGMP 处理和无效的 egl-4 等位基因，鉴定出 cGMP 信号通路的一个组成部分参与感知昆虫信息素 ETDA 的自然变异。我们的数据强烈表明egl-4是涉及太平洋对虾种群行为变异的几个基因座之一。 EGL-4 同源物与其他无脊椎动物模型中的行为变异独立相关，这表明 EGL-4 可能作为跨大系统发育距离的物种间行为库的调节剂。
The geographical mosaic theory of coevolution predicts that different local species interactions will shape population traits, but little is known about the molecular factors involved in mediating the specificity of these interactions. Pristionchus nematodes associate with different scarab beetles around the world, with Pristionchus pacificus isolated primarily from the oriental beetle in Japan. In particular, the constituent populations of. P. pacificus represent a rare opportunity to study multiple specialized interactions and the mechanisms that influence population traits at the genetic level. We identified a component of the cGMP signaling pathway to be involved in the natural variation for sensing the insect pheromone ETDA, using targeted introgression lines, exogenous cGMP treatment, and a null egl-4 allele. Our data strongly implicate egl-4 as one of several loci involved in behavioral variation in P. pacificus populations. That EGL-4 homologs have been independently implicated for behavioral variations in other invertebrate models suggests that EGL-4 may act as a modulator for inter-species behavioral repertoires across large phylogenetic distances.