Analysis of RNA associated with P granules in germ cells of C. elegans adults.
Analysis of RNA associated with P granules in germ cells of C. elegans adults.
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发表时间：
2001-04
期刊：
影响因子：
4.6
通讯作者：
J. A. Schisa;J. Pitt;J. Priess
中科院分区：
文献类型：
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作者：
J. A. Schisa;J. Pitt;J. Priess
P颗粒是一种功能未知的细胞质结构，与线虫性腺中的生殖核相关，在整个生命周期中仅定位于生殖细胞或生殖细胞前体。所有已知的P颗粒的蛋白质组分都含有可能的RNA结合基序，这表明RNA参与了颗粒的结构或功能。然而，在性腺中的P颗粒中还没有发现特异性的mRNAs。我们在这里表明，P颗粒通常含有低水平的RNA，并描述了增加这一水平的条件。我们提供的证据表明，P颗粒中至少存在几种不同的mRNAs，包括pos-1、mex-1、par-3、SKn-1、nos-2和GLD-1mRNA。相反，肌动蛋白、微管蛋白mRNA和rRNA要么不存在于P颗粒中，要么存在于相对较低的水平。我们发现，编码P颗粒蛋白成分的PGL-1和glH(VASA相关)基因家族似乎不是RNA聚集在P颗粒中所必需的；相反，这些蛋白质可能在一些事件中发挥作用，这些事件是RNA从核表面有效运输的先决条件。
P granules are cytoplasmic structures of unknown function that are associated with germ nuclei in the C. elegans gonad, and are localized exclusively to germ cells, or germ cell precursors, throughout the life cycle. All the known protein components of P granules contain putative RNA-binding motifs, suggesting that RNA is involved in either the structure or function of the granules. However, no specific mRNAs have been identified within P granules in the gonad. We show here that P granules normally contain a low level of RNA, and describe conditions that increase this level. We present evidence that several, diverse mRNAs, including pos-1, mex-1, par-3, skn-1, nos-2 and gld-1 mRNA, are present at least transiently within P granules. In contrast, actin and tubulin mRNA and rRNA are either not present in P granules, or are present at relatively low levels. We show that pgl-1 and the glh (Vasa-related) gene family, which encode protein components of P granules, do not appear essential for RNA to concentrate in P granules; these proteins may instead function in events that are a prerequisite for RNAs to be transported efficiently from the nuclear surface.