CELL-SURFACE GLYCOPROTEINS OF HUMAN SARCOMAS - DIFFERENTIAL EXPRESSION IN NORMAL AND MALIGNANT-TISSUES AND CULTURED-CELLS
CELL-SURFACE GLYCOPROTEINS OF HUMAN SARCOMAS - DIFFERENTIAL EXPRESSION IN NORMAL AND MALIGNANT-TISSUES AND CULTURED-CELLS
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DOI：
10.1073/pnas.85.9.3110
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发表时间：
1988-05-01
影响因子：
11.1
通讯作者：
OLD, LJ
中科院分区：
文献类型：
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作者：
RETTIG, WJ;GARINCHESA, P;OLD, LJ
人类细胞的正常分化和恶性转化的特征是表面抗原表型的特异性变化。在本研究中，我们通过使用混合血液吸附测定和免疫化学方法对培养细胞进行分析，并使用免疫组织化学染色对正常组织和> 200个肿瘤标本进行分析，定义了人类肉瘤和正常间充质细胞的六种细胞表面抗原。 F19（Mr，120,000/95,000 糖蛋白）、F24（Mr，95,000 糖蛋白）、G171（Mr 75,000 糖蛋白）、G253（Mr，90,000 糖蛋白）、S5（Mr，120,000 糖蛋白）和 Thy-1（Mr， 25,000 个糖蛋白）抗原表达可表征（i）培养的肉瘤细胞系亚群，（ii）来自各种器官的培养的成纤维细胞，（iii）正常静息和活化的间充质组织，以及（iv）肉瘤和非间充质肿瘤组织。这些结果为培养的间充质细胞和间充质组织提供了基本的表面抗原图谱，并允许根据其抗原表型对人类肉瘤进行分类。
Normal differentiation and malignant transformation of human cells are characterized by specific changes in surface antigen phenotype. In the present study, we have defined six cell-surface antigens of human sarcomas and normal mesenchymal cells, by using mixed hemadsorption assays and immunochemical methods for the analysis of cultured cells and immunohistochemical staining for the analysis of normal tissues and > 200 tumor specimens. Differential patterns of F19 (Mr, 120,000/95,000 glycoprotein), F24 (Mr, 95,000 glycoprotein), G171 (Mr 75,000 glycoprotein), G253 (Mr, 90,000 glycoprotein), S5 (Mr, 120,000 glycoprotein), and Thy-1 (Mr, 25,000 glycoprotein) antigen expression were found to characterize (i) subsets of cultured sarcoma cell lines, (ii) cultured fibroblasts derived from various organs, (iii) normal resting and activated mesenchymal tissues, and (iv) sarcoma and nonmesenchymal tumor tissues. These results provide a basic surface antigenic map for cultured mesenchymal cells and mesenchymal tissues and permit the classification of human sarcomas according to their antigenic phenotypes.