Separation of fatty alcohol ethoxylates by capillary zone electrophoresis and micellar electrokinetic chromatography.
Separation of fatty alcohol ethoxylates by capillary zone electrophoresis and micellar electrokinetic chromatography.
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通过毛细管区带电泳和胶束电动色谱分离脂肪醇乙氧基化物。
DOI：
10.1002/jssc.200800553
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发表时间：
2009
影响因子：
3.1
通讯作者：
K. Otsuka
中科院分区：
文献类型：
--
作者：
R. Koike;F. Kitagawa;K. Otsuka
建立了毛细管区带电泳和胶束电动色谱分离脂肪醇聚氧乙烯醚中环氧乙烷同系物的方法。在分离之前，FAE用2-氟-1-甲基吡啶对甲苯磺酸盐（FMPTS）衍生化以允许CZE分离和UV检测。为了防止在CZE分析中阳离子分析物吸附到毛细管的内表面上并形成胶束，采用含有高浓度乙腈的较低pH背景溶液（BGS）。在最佳条件下，FMPTS衍生的FAE与平均EO数为6在11分钟内完全分离。对于MEKC分析的FAE，十二烷基三甲基氯化铵（DTAC）被添加到BGS。在含有20%（v/v）乙腈的10 mM磷酸盐缓冲液（pH 2.5）中存在30 mM DTAC的情况下，在30 min内实现了含有多达50个EO基团的FAE的上级低聚物分离，具有良好的分析重现性。此外，所开发的方法被应用于分析的商品，如洗衣粉和织物柔软剂中的FAE。
Separation methods based on capillary zone electrophoresis and micellar electrokinetic chromatography were developed to characterize the distribution of ethylene oxide (EO) homologues in the fatty alcohol ethoxylates (FAEs). Prior to the separation, the FAEs were derivatized with 2-fluoro-1-methylpyridinium p-toluenesulfonate (FMPTS) to allow CZE separation and UV detection. To prevent adsorption of cationic analytes onto the inner surface of the capillary and formation of micelles in CZE analysis, a lower pH background solution (BGS) containing a high concentration of acetonitrile was employed. Under optimal conditions, FMPTS-derivatized FAEs with an average EO number of 6 were completely separated within 11 min. For MEKC analysis of the FAEs, dodecyltrimethylammonium chloride (DTAC) was added to the BGS. In the presence of 30 mM DTAC in 10 mM phosphate buffer (pH 2.5) containing 20% (v/v) acetonitrile, superior oligomer separation of the FAEs containing up to 50 EO groups was achieved within 30 min with good analytical reproducibilities. Furthermore, the developed method was applied to the analysis of the FAEs in commercial products such as laundry detergent and fabric softener.