Measurement of the filtration coefficient (Kfc) in the lung of Gallus domesticus and the effects of increased microvascular permeability.
Measurement of the filtration coefficient (Kfc) in the lung of Gallus domesticus and the effects of increased microvascular permeability.
复制标题
鸡肺过滤系数 (Kfc) 的测量以及微血管通透性增加的影响。
DOI：
10.1007/s00360-006-0079-6
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Furlow,JDavid
中科院分区：
文献类型：
--
作者：
Weidner,WJeffrey;Waddell,DavidS;Furlow,JDavid
过滤系数（Kfc）是微血管导电性的敏感指标，已报道许多哺乳动物的肺泡肺，但未报道禽类的支气管旁肺。本研究报告了肯德基在正常鸡的离体肺和给药的鸡肺中的致肿剂油酸（OA）或二甲基酰胺（DMA）。对照Kfc =0.04±0.01 ml min−1kPa−1g−1。在OA（0.12±0.02 ml min - 1kPa - 1g - 1）和DMA（0.07±0.01 ml min - 1kPa - 1g - 1）的作用下，该参数显著增加。由于内皮钙粘蛋白被认为在急性肺损伤的动态反应中发挥作用，我们采用Western blot分析评估肺钙粘蛋白含量，Northern blot分析评估肺血管内皮（VE）钙粘蛋白在给药后的表达。肺钙粘蛋白含量在DMA后明显降低，但OA后没有。VE钙粘蛋白表达增加是DMA治疗的结果，但OA后没有变化。我们的研究结果表明，鸟类肺的通透性特征更接近于哺乳动物而不是爬行动物的肺，并且钙粘蛋白可能在鸟类肺微血管通透性急性增加的反应中起重要作用。
The filtration coefficient (Kfc) is a sensitive measure of microvascular hydraulic conductivity and has been reported for the alveolar lungs of many mammalian species, but not for the parabronchial avian lung. This study reports the Kfc in the isolated lungs of normal chickens and in the lungs of chickens given the edemogenic agents oleic acid (OA) or dimethyl amiloride (DMA). The control Kfc =0.04±0.01 ml min−1kPa−1g−1. This parameter increased significantly following the administration of both OA (0.12±0.02 ml min−1kPa−1g−1) and DMA (0.07±0.01 ml min kPa−1g−1). As endothelial cadherins are thought to play a role in the dynamic response to acute lung injury, we utilized Western blot analysis to assess lung cadherin content and Northern blot analysis to assess pulmonary vascular endothelial (VE) cadherin expression following drug administration. Lung cadherin content decreases markedly following DMA, but not OA administration. VE cadherin expression increases as a result of DMA treatment, but is unchanged following OA. Our results suggest that the permeability characteristics of the avian lung are more closely consistent with those of the mammalian rather than the reptilian lung, and, that cadherins may play a significant role in the response to acute increases in avian pulmonary microvascular permeability.