ATPase activity of SopA, a protein essential for active partitioning of F plasmid
ATPase activity of SopA, a protein essential for active partitioning of F plasmid
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SopA 的 ATP 酶活性，SopA 是 F 质粒主动分配所必需的蛋白质
DOI：
10.1007/bf00538693
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
K. Nagai
中科院分区：
文献类型：
--
作者：
Eijiro Watanabe;M. Wachi;M. Yamasaki;K. Nagai
摘要F质粒的SOPA、B、C基因在大肠杆菌细胞分裂过程中起着至关重要的作用。本文对SOPA和SopB基因产物进行了分离，并对其生化活性进行了研究。[α-32P]ATP在紫外光照射下与SOPA蛋白发生交联；只有在镁离子存在的情况下才能观察到这种交联，而在非放射性的三磷酸腺苷、二磷酸腺苷和二磷酸腺苷的存在下，这种交联会被竞争性地抑制，而其他的三磷酸腺苷或二磷酸核苷则不能。相反，没有检测到SopB蛋白的ATP结合活性。SOPA蛋白表现出适度的镁离子依赖的ATPase活性，这种活性在DNA存在下被刺激。在DNA存在的情况下，SopB蛋白的加入进一步刺激了ATPase的活性。然而，单独的SopB蛋白不能刺激ATPase的活性。
SummaryThe SopA, B, C genes of the F plasmid play an essential role in plasmid partitioning during cell division in Escherichia coli. In this paper, the products of the sopA and sopB genes were isolated and their biochemical activities studied. [α-32P]ATP was cross-linked to the SopA protein by UV irradiation; this cross-linking was observed only in the presence of magnesium ion, and was competitively inhibited in the presence of non-radioactive ATP, ADP and dATP, but not other NTPs or dNTPs. In contrast, no ATP binding activity was detected for the SopB protein. The SopA protein showed a modest magnesium ion-dependent ATPase activity and this activity was stimulated in the presence of DNA. The ATPase activity in the presence of DNA was further stimulated by addition of the SopB protein. However, the SopB protein alone failed to stimulate the ATPase activity.