The Use of Antisense Oligonucleotides in Evaluating Survivin as a Therapeutic Target for Radiation Sensitization in Lung Cancer.
The Use of Antisense Oligonucleotides in Evaluating Survivin as a Therapeutic Target for Radiation Sensitization in Lung Cancer.
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DOI：
10.1251/bpo95
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发表时间：
2004
影响因子：
6.4
通讯作者：
Lu B
中科院分区：
文献类型：
--
作者：
Shinohara ET;Hallahan DE;Lu B
阐明蛋白质过度表达和表达不足的机制对于更好地理解癌症至关重要。多种技术用于检查细胞中蛋白质的差异表达，并评估蛋白质表达响应于治疗（如放射）的变化。表达减少可由蛋白质失活、mRNA不稳定性或转录减少引起。以下方案用于确定抗凋亡因子存活素在正常组织中响应于辐射的表达降低的机制以及阻止这种降低的癌细胞缺陷。我们还研究了克服癌细胞中生存素过度表达的方法，以使它们对辐射敏感。我们将集中在使用反义寡核苷酸，细胞周期分析和荧光素酶报告基因。
Elucidating the mechanism of over and under expression of proteins is critical in developing a better understanding of cancer. Multiple techniques are used to examine differential expression of proteins in cells and assess changes in protein expression in response to therapies such as radiation. Reduced expression can be caused by protein inactivation, mRNA instability, or reduced transcription. The following protocol was used to determine the mechanism for the reduced expression of an antiapoptotic factor, survivin, in normal tissues in response to radiation and the defect in cancer cells that prevents this reduction. We also examined ways to overcome survivin over expression in cancer cells in order to sensitize them to radiation. We will focus on the use of antisense oligonucleotides, cell cycle analysis, and luciferase reporter genes.