Two-dimensional mass spectrometric mapping.
Two-dimensional mass spectrometric mapping.
复制标题
二维质谱图。
DOI：
10.1016/j.cbpa.2005.12.017
复制
发表时间：
2006
影响因子：
7.8
通讯作者：
D. Neri
中科院分区：
文献类型：
--
作者：
C. Roesli;G. Elia;D. Neri
密切相关的生物样品中蛋白质的鉴定和相对定量是系统生物学许多研究和生物标志物发现的支柱。虽然基于二维凝胶的方法仍然广泛用于比较蛋白质组学研究，但最近出现的无凝胶方法可能允许以自动化方式分析大量样品。本综述中介绍的大多数技术都需要在分析前对蛋白质进行化学修饰，并依赖质谱信号的相对强度进行蛋白质定量。特别是，二维质谱图谱方法提供了质谱数据的可视化表示，从而有助于识别相对蛋白质丰度的差异。
The identification and relative quantification of proteins in closely related biological samples is the backbone for many investigations in systems biology and for the discovery of biomarkers. While two-dimensional gel-based methodologies are still widely used for comparative proteomic studies, the recent advent of gel-free methodologies may allow the analysis of a larger number of samples in an automated fashion. Most of the technologies presented in this review require a chemical modification of proteins before analysis, and rely on the relative intensities of mass spectrometry signals for protein quantification. In particular, two-dimensional mass spectrometric mapping methodologies provide a visual representation of mass spectrometric data, thus facilitating the identification of differences in relative protein abundance.
影响因子：
4.4
作者：
Zang, L;Toy, DP;Karger, BL
通讯作者：
Karger, BL