Ciprofloxacin-Resistant Staphylococcus aureus Displays Enhanced Resistance and Virulence in Iron-Restricted Conditions
Ciprofloxacin-Resistant Staphylococcus aureus Displays Enhanced Resistance and Virulence in Iron-Restricted Conditions
复制标题
耐环丙沙星金黄色葡萄球菌在缺铁条件下表现出增强的耐药性和毒力
DOI：
10.1021/acs.jproteome.1c00077
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发表时间：
2021-04-19
影响因子：
4.4
通讯作者：
Sun, Xuesong
中科院分区：
文献类型：
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作者：
Dong, Yingshan;Miao, Xinyu;Sun, Xuesong
抗生素的不合理滥用导致了大规模耐药菌的出现，严重威胁人类健康。与药敏菌相比，耐药菌很难被宿主免疫力清除。为了充分探讨耐药菌对铁限制环境的适应机制，我们对环丙沙星（CIP）耐药金黄色葡萄球菌（CIP-R）在铁存在或不存在的情况下进行了基于数据独立采集的定量蛋白质组学研究。生物信息学分析表明，CIP-R菌具有较强的氨基酸合成和能量储存能力。值得注意的是，CIP-R细菌通过在铁限制胁迫下上调许多毒力相关蛋白的表达和增强毒力相关氨基酸的合成来增加毒力。这项研究将有助于我们进一步解释导致细菌对抗生素耐药性依赖于宿主环境的适应机制，并为开发治疗耐药细菌感染的新药提供见解。
The unreasonable misuse of antibiotics has led to the emergence of large-scale drug-resistant bacteria, seriously threatening human health. Compared with drug-sensitive bacteria, resistant bacteria are difficult to clear by host immunity. To fully explore the adaptive mechanism of resistant bacteria to the iron-restricted environment, we performed data-independent acquisition-based quantitative proteomics on ciprofloxacin (CIP)-resistant (CIP-R) Staphylococcus aureus in the presence or absence of iron. On bioinformatics analysis, CIP-R bacteria showed stronger amino acid synthesis and energy storage ability. Notably, CIP-R bacteria increased virulence by upregulating the expression of many virulence-related proteins and enhancing the synthesis of virulence-related amino acids under iron-restricted stress. This study will help us to further explain the adaptive mechanisms that lead to bacterial resistance to antibiotics depending on the host environment and provide insights into the development of novel drugs for the treatment of drug-resistant bacterial infections.