Nerve growth factor gene expression in the developing rat brain.
Nerve growth factor gene expression in the developing rat brain.
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DOI：
10.1126/science.3764415
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发表时间：
1986-10
期刊：
影响因子：
56.9
通讯作者：
T. Large;S. Bodary;D. Clegg;G. Weskamp;U. Otten;L. Reichardt
中科院分区：
文献类型：
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作者：
T. Large;S. Bodary;D. Clegg;G. Weskamp;U. Otten;L. Reichardt
本实验研究了神经生长因子（NGF）蛋白及其信使RNA（mRNA）在大鼠脑发育过程中的调节作用，以验证NGF支持基底前脑胆碱能神经元分化的假说。在成年人中，这些神经元的主要目标，海马和新皮层，含有最高浓度的NGF mRNA，但相对较低的比率的NGF蛋白质，其mRNA。与此相反，高浓度的NGF蛋白和低浓度的NGFmRNA被认为是在基底前脑，与NGF蛋白从新皮层和海马到该区域的逆行运输相一致。在这两个目标区域，NGF和NGF mRNA在出生时几乎检测不到，它们的浓度在第21天增加到峰值，然后NGF mRNA，但不是NGF蛋白，到第35天下降了三倍。NGF在基底前脑中的积累超过了在靶区域中的积累，并且先于胆碱乙酰转移酶的增加，这表明胆碱能投射神经元的分化确实受到逆行转运的NGF的调节。此外，高比例的NGF蛋白质的NGFmRNA，在基底前脑，被认为是在嗅球和小脑，这表明，NGF可能被运送到这些地区的身份不明的神经元。
The regulation of nerve growth factor (NGF) protein and NGF messenger RNA (mRNA) in the developing rat brain has been studied to assess the hypothesis that NGF supports the differentiation of cholinergic neurons in the basal forebrain. In the adult, the major targets of these neurons, the hippocampus and neocortex, contain the highest concentrations of NGF mRNA, but comparatively low ratios of NGF protein to its mRNA. In contrast, a high concentration of NGF protein and a low concentration of NGF mRNA were seen in the basal forebrain, consistent with retrograde transport of NGF protein into this region from the neocortex and hippocampus. In these two target regions NGF and NGF mRNA were barely detectable at birth, their concentrations increased to a peak at day 21, and then NGF mRNA, but not NGF protein, declined threefold by day 35. NGF accumulation in the basal forebrain paralleled that in the target regions and preceded an increase in choline acetyltransferase, suggesting that the differentiation of cholinergic projection neurons is indeed regulated by retrogradely transported NGF. In addition, high ratios of NGF protein to NGF mRNA, comparable to that in the basal forebrain, were seen in the olfactory bulb and cerebellum, suggesting that NGF may be transported into these regions by unidentified neurons.