The oyster genome reveals stress adaptation and complexity of shell formation
The oyster genome reveals stress adaptation and complexity of shell formation
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DOI：
10.1038/nature11413
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发表时间：
2012-10-04
期刊：
影响因子：
64.8
通讯作者：
Wang, Jun
中科院分区：
文献类型：
--
作者：
Zhang, Guofan;Fang, Xiaodong;Wang, Jun
太平洋牡蛎Crassostrea gigas属于物种最丰富但基因组研究很少的门之一，软体动物门。在这里，我们报告的牡蛎基因组的测序和组装使用短读段和一个fosmid池策略，沿着与转录组的发展和压力反应和蛋白质组的外壳。牡蛎的基因组是高度多态性和丰富的重复序列，与一些转座因子仍然积极塑造变化。转录组研究揭示了一组广泛的基因对环境压力的反应。编码热休克蛋白70和细胞凋亡抑制剂的基因的扩展可能是牡蛎适应高度紧张的潮间带固着生活的核心。我们的分析还表明，软体动物中的壳形成比目前理解的更复杂，涉及细胞及其外泌体的广泛参与。牡蛎基因组序列填补了我们对Lophotrochozoa理解的空白。
The Pacific oyster Crassostrea gigas belongs to one of the most species-rich but genomically poorly explored phyla, the Mollusca. Here we report the sequencing and assembly of the oyster genome using short reads and a fosmid-pooling strategy, along with transcriptomes of development and stress response and the proteome of the shell. The oyster genome is highly polymorphic and rich in repetitive sequences, with some transposable elements still actively shaping variation. Transcriptome studies reveal an extensive set of genes responding to environmental stress. The expansion of genes coding for heat shock protein 70 and inhibitors of apoptosis is probably central to the oyster's adaptation to sessile life in the highly stressful intertidal zone. Our analyses also show that shell formation in molluscs is more complex than currently understood and involves extensive participation of cells and their exosomes. The oyster genome sequence fills a void in our understanding of the Lophotrochozoa.