Recombinant Phage Elicits Protective Immune Response against Systemic S. globosa Infection in Mouse Model.
Recombinant Phage Elicits Protective Immune Response against Systemic S. globosa Infection in Mouse Model.
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DOI：
10.1038/srep42024
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发表时间：
2017-02-06
影响因子：
4.6
通讯作者：
Wang L
中科院分区：
文献类型：
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作者：
Chen F;Jiang R;Wang Y;Zhu M;Zhang X;Dong S;Shi H;Wang L
全球孢子丝菌是一种真菌，通常感染免疫功能低下的患者。它的预防仍然是一项挑战。一种70 kda的孢丝菌糖蛋白（Gp70）曾被报道保护宿主免受这种真菌的感染。在这里，我们在主要外壳蛋白（pIII）上显示了Gp70的表位肽（kpvqhalltplgldr），并研究了其作为预防全球葡萄球菌感染的疫苗的有效性。用重组噬菌体和热灭活的球状葡萄球菌分别免疫小鼠。在本研究中，我们评估了小鼠的体液和细胞免疫反应，证明重组噬菌体可以诱导小鼠产生更强的免疫反应，并产生抑制全球葡萄球菌感染的抗体。此外，用重组噬菌体免疫可以提高全球葡萄球菌感染小鼠的存活率。这些结果表明，kvqhalltplgldr重组噬菌体是一种潜在的抗全球葡萄球菌感染的候选疫苗。
Sporothrix globosa is a type of fungus that typically infects immunocompromised patients. Its prevention continues to pose a challenge. A 70-KDa glycoprotein (Gp70) of Sporothrix has been previously reported to protect host against infection from this fungus. Here, we displayed an epitope peptide (kpvqhalltplgldr) of Gp70 on the major coat protein (pIII), and investigated its efficiency as a vaccine for preventing S. globosa infection. The recombinant phage and the heat-killed S. globosa were used to immunize mice separately. In this study, we evaluated the humoral and cellular immune responses in the mice and demonstrated that recombinant phage could induce mice to produce a stronger immune response and generate antibodies to inhibit S. globosa infection. Furthermore, immunization with recombinant phage could increase the survival rate of S. globosa infection in mice. All these results together indicated that recombinant phages displaying kpvqhalltplgldr are a potential vaccine candidate against S. globosa infection.