Spatial pattern and temporal evolution of retinal oxygenation response in oxygen-induced retinopathy.
Spatial pattern and temporal evolution of retinal oxygenation response in oxygen-induced retinopathy.
复制标题
氧诱导性视网膜病变中视网膜氧合反应的空间模式和时间演变。
DOI：
10.1167/iovs.03-0415
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发表时间：
2003
影响因子：
4.4
通讯作者：
Berkowitz,BruceA
中科院分区：
文献类型：
--
作者：
Roberts,Robin;Zhang,Wei;Ito,Yasuki;Berkowitz,BruceA
目的。确定与视网膜新生血管（NV）小鼠氧诱导视网膜病变（OIR）模型相关的视网膜氧分压（ΔP o 2）变化的空间模式和时间演变。方法。在 P7 时，新生 C57BL/6 小鼠暴露于 75% 的氧气中，直到出生后第 12 天 (P)，然后在室内空气中恢复，直到 P17 或 P34。对照小鼠留在室内空气中直到 P17 或 P34。在 P17 和 P34 时，使用功能性磁共振成像 (MRI) 和卡波金吸入激发来测量视网膜 ΔP o 2。在用腺苷二磷酸酶 (ADPase) 染色的视网膜中测量视网膜无血管性、视神经乳头到视网膜周边血管边缘的距离以及 NV 发生率和严重程度。结果。在 P17 和 P34 对照以及 P34 OIR 动物中，视网膜完全血管化，没有 NV 的证据。在 P17 OIR 小鼠中，100%（36/36）的小鼠在周围血管和中央毛细血管视网膜的边界处存在大的中央视网膜毛细血管无区（整个视网膜区域的 22%±3%，平均值±SD）和 4 小时（范围 1-7）的视网膜 NV。在P17 OIR小鼠中，血管化的远周边视网膜上的视网膜ΔP o 2 与P17对照没有显着差异（P>0.05），但在中央无毛细血管的视网膜上是超正常的（P<0.05）。然而，P34对照组和OIR组之间的视网膜ΔP o 2 没有发现差异(P>0.05)。结论。在小鼠 OIR 模型中视网膜 NV 出现期间，仅在中央毛细血管视网膜上发现了可逆的超常 ΔP o 2 。目前的数据显示了碳素挑战功能性 MRI 对眼睛体内视网膜 ΔP o 2 的研究的适用性，因为眼睛太小，无法使用现有技术。
purpose. To determine the spatial pattern and temporal evolution of the change in retinal partial oxygen pressure (ΔP o 2) associated with a murine oxygen-induced retinopathy (OIR) model of retinal neovascularization (NV).methods. On P7, newborn C57BL/6 mice were exposed to 75% oxygen until postnatal day (P) 12, followed by recovery in room air until P17 or P34. Control mice remained in room air until P17 or P34. At P17 and P34, functional magnetic resonance imaging (MRI) and a carbogen inhalation challenge was used to measure retinal ΔP o 2. Retinal avascularity, distance from the optic nerve head to the vascular edge in the peripheral retina, and NV incidence and severity were measured in retinas stained with adenosine diphosphatase (ADPase).results. In P17 and P34 controls and in P34 OIR animals, retinas were fully vascularized without evidence of NV. In P17 OIR mice, there was a large central retinal capillary-free zone (22%±3% of the entire retinal area, mean±SD) and 4 clockhours (range 1–7) of retinal NV at the border of the peripheral vascular and central acapillary retina in 100%(36/36) of the mice. In P17 OIR mice, retinal ΔP o 2 over the vascularized far peripheral retina was not significantly (P> 0.05) different from the P17 control but was supernormal (P< 0.05) over the central capillary-free retina. However, no differences (P> 0.05) in retinal ΔP o 2 were found between the P34 control and OIR groups.conclusions. A reversible supernormal ΔP o 2 was found only over the central acapillary retina during the appearance of retinal NV in a mouse OIR model. The present data show the applicability of carbogen-challenge functional MRI to the study of retinal ΔP o 2 in vivo in eyes that are too small for the use of existing techniques.